1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Protein Tyrosine Kinases
  4. Activated Cdc42-Associated Kinase 1 (ACK1)
  5. ACK1 Protein, Human (sf9, GST)

ACK1 Protein, Human (sf9, GST)

Cat. No.: HY-P75542
COA Handling Instructions

The ACK1 protein is a non-receptor tyrosine protein and serine/threonine protein kinase that orchestrates cellular physiology by affecting cell spreading, migration, survival, growth, and proliferation. It phosphorylates AKT1, AR, MCF2, WASL and WWOX, affecting various cellular processes. ACK1 Protein, Human (sf9, GST) is the recombinant human-derived ACK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of ACK1 Protein, Human (sf9, GST) is 367 a.a., with molecular weight of ~62 kDa.

For research use only. We do not sell to patients.

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Description

The ACK1 protein is a non-receptor tyrosine protein and serine/threonine protein kinase that orchestrates cellular physiology by affecting cell spreading, migration, survival, growth, and proliferation. It phosphorylates AKT1, AR, MCF2, WASL and WWOX, affecting various cellular processes. ACK1 Protein, Human (sf9, GST) is the recombinant human-derived ACK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of ACK1 Protein, Human (sf9, GST) is 367 a.a., with molecular weight of ~62 kDa.

Background

ACK1 Protein, a non-receptor tyrosine-protein and serine/threonine-protein kinase, plays a multifaceted role in cell physiology, influencing cell spreading, migration, survival, growth, and proliferation by transducing extracellular signals to cytosolic and nuclear effectors. It phosphorylates AKT1, AR, MCF2, WASL, and WWOX, impacting various cellular processes. Involved in trafficking and clathrin-mediated endocytosis, ACK1 binds to epidermal growth factor receptor (EGFR) and clathrin, regulating ligand-induced EGFR degradation and contributing to EGFR accumulation at early endosomes' limiting membrane. As a downstream effector of CDC42, ACK1 mediates CDC42-dependent cell migration through BCAR1 phosphorylation. In brain development and adult synaptic function, ACK1 may play a role. Activating AKT1 by phosphorylating it on 'Tyr-176,' ACK1 also phosphorylates AR on 'Tyr-267' and 'Tyr-363,' promoting its recruitment to androgen-responsive enhancers (AREs). Additionally, ACK1 phosphorylates WWOX on 'Tyr-287' and MCF2, enhancing its guanine nucleotide exchange factor (GEF) activity towards Rho family proteins. Furthermore, ACK1 contributes to the control of AXL receptor levels. In cancer, ACK1 confers metastatic properties and promotes tumor growth by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors including AKT1 and AR, showcasing its intricate involvement in oncogenic processes. Additionally, ACK1 phosphorylates WASP, further underscoring its diverse signaling capabilities.

Biological Activity

The specific activity was determined to be ≥ 4 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

Q07912-1 (G110-W476)

Gene ID
Molecular Construction
N-term
GST
ACK1 (G110-W476)
Accession # Q07912-1
C-term
Synonyms
Activated CDC42 kinase 1; Tyrosine kinase non-receptor protein 2; TNK2
Molecular Weight

Approximately 62 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 0.5 mM EDTA, 0.5 mM PMSF, 0.5 mM TCEP.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
ACK1 Protein, Human (sf9, GST)
Cat. No.:
HY-P75542
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