ACK1 Protein, Human (Active, sf9, GST)

ACK1 Protein, a non-receptor tyrosine-protein and serine/threonine-protein kinase, plays a multifaceted role in cell physiology, influencing cell spreading, migration, survival, growth, and proliferation by transducing extracellular signals to cytosolic and nuclear effectors. It phosphorylates AKT1, AR, MCF2, WASL, and WWOX, impacting various cellular processes. Involved in trafficking and clathrin-mediated endocytosis, ACK1 binds to epidermal growth factor receptor (EGFR) and clathrin, regulating ligand-induced EGFR degradation and contributing to EGFR accumulation at early endosomes' limiting membrane. As a downstream effector of CDC42, ACK1 mediates CDC42-dependent cell migration through BCAR1 phosphorylation. In brain development and adult synaptic function, ACK1 may play a role. Activating AKT1 by phosphorylating it on 'Tyr-176,' ACK1 also phosphorylates AR on 'Tyr-267' and 'Tyr-363,' promoting its recruitment to androgen-responsive enhancers (AREs). Additionally, ACK1 phosphorylates WWOX on 'Tyr-287' and MCF2, enhancing its guanine nucleotide exchange factor (GEF) activity towards Rho family proteins. Furthermore, ACK1 contributes to the control of AXL receptor levels. In cancer, ACK1 confers metastatic properties and promotes tumor growth by negatively regulating tumor suppressors such as WWOX and positively regulating pro-survival factors including AKT1 and AR, showcasing its intricate involvement in oncogenic processes. Additionally, ACK1 phosphorylates WASP, further underscoring its diverse signaling capabilities.