ACOT13 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ACOT13 Protein, Human (HEK293, His) is human recombinant Acyl-coenzyme A thioesterase 13 (ACOT13) with a N-terminal His-tag. ACOT13 Protein, Human (HEK293, His) contains 160 amino acids (1-140 a.a.).
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
ACOT13 Protein, Human (HEK293, His) is human recombinant Acyl-coenzyme A thioesterase 13 (ACOT13) with a N-terminal His-tag. ACOT13 Protein, Human (HEK293, His) contains 160 amino acids (1-140 a.a.).
Background
Acyl-coenzyme A thioesterase 13 (ACOT13), also known as thioesterase superfamily member 2 (Them2), belongs to the mammalian acyl-CoA thioesterase family. ACOT13 is a homotetrameric hotdog fold thioesterase, and is enriched in oxidative tissues, associated with mitochondria, and relatively specific for long chain fatty acyl-CoA substrates. ACOT13 plays a key role in hepatic lipid and glucose metabolism. ACOT13 is essential for the cell sustained proliferation and is co-localized with microtubules[1][2].
Verified Bioactivity
Measured by its ability to Hydrolysis 500μg/mL Myristoyl coenzyme A lithium and generate product of TNB. The specific activity is 6.66 pmol/min/µg that incubate at 25 ℃ in kinetic mode for 60 minutes.
Assay Procedure
Materials
Assay Buffer: 50 mM KCl, 10 mM Hepes and 0.3 mM DTNB , pH 7.5
Substrate: Myristoyl coenzyme A lithium (HY-126833A)
ACOT13 Protein, Human (HEK293, His) (HY-P7452)
Standard: TNB
Procedure
1. Dilute Human ACOT13 to 100 μg/mL in experimental buffer.
2. Dilute the substrate to 1 mg/mL in the experimental buffer.
3. Experimental group: 50 μL Human ACOT13 protein + 50 μL substrate.
Blank group: 50 μL experimental buffer + 50 μL substrate.
4. Determination of OD value at 412 nm for 1 hour under kinetic mode.
5. Preparation of standard curve: Dilute TNB with experimental buffer to a final concentration of 0, 0.0001, 0.001, 0.01, 0.1, 1 mM, 100 μL per well was added into 96-well plate, and OD412 was measured in endpoint mode.
6. Calculate specific activity:
Specific Activity (pmol/min/μg) = | Adjusted Vmax* (RFU/min) x Conversion Factor ** (pmol/RFU) |
| amount of enzyme (μg) |
*Adjusted for Control
**Derived using calibration standard
Per Well:
Human ACOT13: 5 μg
Substrate: 0.5 mg/mL
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q9NPJ3-1 (T2-N140)
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Molecular Construction
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N-term
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ACOT13 (T2-N140)
Accession # Q9NPJ3-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
ACOT13; Hotdog-Fold Thioesterase Superfamily Member 2; Prev. THEM2; Palmitoyl-CoA Hydrolase; Thioesterase Superfamily Member 2; Hypothalamus Protein HT012; HT012; PNAS-27; Acyl-CoA Thioesterase 13; Acyl-Coenzyme A Thioesterase 13
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AA Sequence
TSMTQSLREVIKAMTKARNFERVLGKITLVSAAPGKVICEMKVEEEHTNAIGTLHGGLTATLVDNISTMALLCTERGAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN
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Predicted Molecular Mass
15.9 kDa
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Molecular Weight
Approximately 15 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 8% Sucrose, 100 mM NaCl, 0.05% Tween 80, pH 7.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Wei J, et al. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem J. 2009 Jun 26;421(2):311-22. [Content Brief]
[2]. Cheng Z, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem Biophys Res Commun. 2006 Dec 1;350(4):850-3. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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