Activin A Protein, Human/Mouse/Rat (CHO)

The INHBA protein plays a key role in regulating pituitary function by regulating follicle-stimulating hormone secretion together with activin. Its broad effects span a variety of physiological processes, including hormone secretion, germ cell development, erythrocyte differentiation, insulin secretion, nerve cell survival, embryonic development, and bone growth, depending on unique subunit composition. Activin A Protein, Human/Mouse/Rat (CHO) is the recombinant human/rat/mouse-derived Activin A protein, expressed by CHO, with tag free.

INHBA protein assumes a pivotal role in the intricate regulation of pituitary gland function, contributing to the opposing dynamics of inhibiting and activating follitropin secretion alongside activins. The expansive influence of inhibins and activins, with INHBA as a central player, spans a spectrum of physiological processes, including hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development, and bone growth, contingent upon their unique subunit compositions. Notably, inhibins, such as Inhibin A and Inhibin B, emerge as counterparts opposing the functions of activins. Structurally, INHBA exists in a dimeric form, intricately linked by one or more disulfide bonds, representing a homodimer of beta-A subunits. The diversity of activins, encompassing Activin A, Activin B, and Activin AB, further emphasizes their specific subunit compositions, influencing interactions with regulatory proteins like FST and FSTL3. This intricate interplay underscores INHBA's central role in orchestrating a finely tuned regulatory network governing diverse physiological functions.

1.Measured by its binding ability in a functional ELISA.Immobilized Human Activin A Protein, premium grade at 5 μg/mL (100 μL/well) can bind Biotinylated Human Activin RIIB Protein, His, Avitag with the EC₅₀ of 2.72 ng/ml.
2.Human Activin A Protein, premium grade stimulates Human Activin RIl (Luc) HEK293 Reporter Cell. The specific activity of Human Activin A Protein, premium grade is ＞5.00 x 10² IU/mg, which is calibrated against WHO Reference Reagent Activin A (Human, Recombinant) NIBSC code: 91/626.

Rat; Mouse; Human

CHO

Tag Free

P08476 (G311-S426)

3624

Full Length of Mature Protein

GLECDGKVNICCKKQFFVSFKDIGWNDWIIAPSGYHANYCEGECPSHIAGTSGSSLSFHSTVINHYRMRGHSPFANLKSCCVPTKLRPMSMLYYDDGQNIIKKDIQNMIVEECGCS

Approximately 10-16 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 with 11% trehalose as protectant.

<0.01 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.