ALOX12 Protein, Human (His)
Based on 1 publication(s) in Google Scholar
ALOX12 Protein, Human (His) is a recombinant ALOX12 protein with a His tag. ALOX12 encodes arachidonic acid 12-lipoxygenase that acts on different polyunsaturated fatty acid substrates to produce biologically active lipid mediators including eicosanes and lipoxins.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
ALOX12 Protein, Human (His) is a recombinant ALOX12 protein with a His tag. ALOX12 encodes arachidonic acid 12-lipoxygenase that acts on different polyunsaturated fatty acid substrates to produce biologically active lipid mediators including eicosanes and lipoxins[1].
Background
ALOX12 mainly metabolizes arachidonic acid to 12S-hydroperoxyeicosatetraenoic acid (12(S)-HpETE) and further reduces it to 12(S)-HETE by peroxyglutathione. ALOX12 is widely expressed in various cell types. Because ALOX12 has the function of regulating platelet aggregation, cell migration, and tumor cell proliferation, it is mainly involved in the development of diseasessuch as thrombosis, atherosclerosis, and cancer. ALOX12 is involved in the regulation of inflammation and apoptosisand is involved in the development of liver injury through the activation of the NF-kB signaling pathway and Caspase-3. ALOX12 plays an important role in the occurrence and development of diseases.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Publications (1)
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Journal Impact Factor
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Most Recent
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Phytomedicine
Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting ALOX12-dependent ferroptosis. [Abstract]2024 Jul 25:130:155757. PMID: 38805781
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P18054 (M1-I663)
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Molecular Construction
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N-term
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6*His
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ALOX12 (M1-I663)
Accession # P18054 -
C-term
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Protein Length
Full Length
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Synonyms
ALOX12; Platelet 12-LOX; Arachidonate 12-Lipoxygenase, 12S Type; LOG12; 12S-LOX; Arachidonate 15-Lipoxygenase,15S-Type; Polyunsaturated Fatty Acid Lipoxygenase ALOX12; Platelet-Type 12-Lipoxygenase; Arachidonate 12-Lipoxygenase, 12S-Type; Arachidonate 12-Lipoxygenase; Arachidonate (12S)-Lipoxygenase; Linoleate (13S)-Lipoxygenase; Arachidonate (15S)-Lipoxygenase; Linoleate 13S-Lipoxygenase; Platelet-Type Lipoxygenase 12; 12-LOX; Lipoxin Synthase 12-LO; 12LO; 12S-Lipoxygenase
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AA Sequence
MGRYRIRVATGAWLFSGSYNRVQLWLVGTRGEAELELQLRPARGEEEEFDHDVAEDLGLLQFVRLRKHHWLVDDAWFCDRITVQGPGACAEVAFPCYRWVQGEDILSLPEGTARLPGDNALDMFQKHREKELKDRQQIYCWATWKEGLPLTIAADRKDDLPPNMRFHEEKRLDFEWTLKAGALEMALKRVYTLLSSWNCLEDFDQIFWGQKSALAEKVRQCWQDDELFSYQFLNGANPMLLRRSTSLPSRLVLPSGMEELQAQLEKELQNGSLFEADFILLDGIPANVIRGEKQYLAAPLVMLKMEPNGKLQPMVIQIQPPNPSSPTPTLFLPSDPPLAWLLAKSWVRNSDFQLHEIQYHLLNTHLVAEVIAVATMRCLPGLHPIFKFLIPHIRYTMEINTRARTQLISDGGIFDKAVSTGGGGHVQLLRRAAAQLTYCSLCPPDDLADRGLLGLPGALYAHDALRLWEIIARYVEGIVHLFYQRDDIVKGDPELQAWCREITEVGLCQAQDRGFPVSFQSQSQLCHFLTMCVFTCTAQHAAINQGQLDWYAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRRQPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYEYLKPSCIENSVTI
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Predicted Molecular Mass
79.8 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6%
trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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