Alpha-Fetoprotein Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
Alpha-FetoProtein, Mouse (HEK293, His) is a mouse recombinant AFP with a His tag at the C-terminus. Alpha-FetoProtein, Mouse (HEK293, His) is produced by Mammalian expression system and the target gene encoding Lys19-Val605 is expressed.
- Species: Mouse
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Alpha-FetoProtein, Mouse (HEK293, His) is a mouse recombinant AFP with a His tag at the C-terminus. Alpha-FetoProtein, Mouse (HEK293, His) is produced by Mammalian expression system and the target gene encoding Lys19-Val605 is expressed.
Background
Alpha-fetoprotein (AFP) is a serum glycoprotein through receptor-mediated endocytosis to embryotic cells. AFP is a marker to pathological conditions including neoplastic and non-neoplastic diseases, antioxidant effects, growth regulator in different cells and in cancer, immune response modulator, and carrier for fatty acids and oestrogens. Toxins delivered by AFP can damage myeloid-derived suppressor cells and consequently unleash innate and adaptive immunity to destroy cancer cells[1][2].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Mouse
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Source HEK293
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Tag C-His
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Accession
P02772 (K19-V605)
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Molecular Construction
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N-term
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Alpha-Fetoprotein (K19-V605)
Accession # P02772 -
His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
AFP; Alpha-Fetoglobulin; Prev. HPAFP; Fetoglobulin; Alpha-Fetoprotein; AFPD; Alpha-1-Fetoprotein; Alpha Fetoprotein; FETA
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AA Sequence
KALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDVLAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEPAESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELREGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKVMTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASFLHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVNSGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELTEEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV
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Molecular Weight
Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (264 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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