Alkaline Phosphatase/ALPP Protein, Human (HEK293, His, solution)
Based on 1 Customer Validation
Alkaline Phosphatase/ALPP Protein, Human (HEK293, His, solution) is a ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
Alkaline Phosphatase/ALPP Protein, Human (HEK293, His, solution) is a ubiquitous membrane-bound glycoprotein that catalyzes the hydrolysis of phosphate monoesters at basic pH values[1].
Background
Alkaline phosphatases [ALP; orthophosphoric monoester phosphohydrolase (alkaline optimum) EC 3.1.3.1] are plasma membrane-bound glycoproteins. These enzymes are widely distributed in nature, including prokaryotes and higher eukaryotes, with the exception of some higher plants. Alkaline phosphatase forms a large family of dimeric enzymes, usually confined to the cell surface hydrolyzes various monophosphate esters at a high pH optimum with release of inorganic phosphate [1].
Verified Bioactivity
Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP). The specific activity is 672399.07 pmol/min/µg, as measured under the described conditions.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P05187 (I23-D506)
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Molecular Construction
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N-term
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ALPP (I23-D506)
Accession # P05187 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ALPP; PLAP-1; Alkaline Phosphatase, Placental; Alkaline Phosphatase, Placental (Regan Isozyme); PLAP; Placental Alkaline Phosphatase; PALP; Alkaline Phosphomonoesterase; Alkaline Phosphatase, Placental Type; Glycerophosphatase; Alkaline Phosphatase Regan
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AA Sequence
IIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
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Predicted Molecular Mass
53.7 kDa
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Molecular Weight
Approximately 60-85 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filter solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (264 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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