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  4. AMIGO2 Protein, Human (HEK293, His)

AMIGO2 Protein, Human (HEK293, His)

Cat. No.: HY-P7495
COA Handling Instructions

AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane.

For research use only. We do not sell to patients.

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10 μg $160 In-stock
Estimated Time of Arrival: December 31
50 μg $490 In-stock
Estimated Time of Arrival: December 31
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  • Biological Activity

  • Technical Parameters

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Description

AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane[1].

Background

AMIGO2 is a scaffold protein that regulates PDK1 membrane localization and Akt activation. This protein contains six leucine-rich repeats (LRRs), a single IgG-like domain, a transmembrane domain, and a cytosolic domain. AMIGO2 plays its role by controling PDK1 localization to the plasma membrane and then directly interacts with PDK1 to activate the Akt pathway[1].
Loss of AMIGO2 in endothelial cells (ECs) leads to apoptosis and inhibition of angiogenesis with Akt inactivation[1].
AMIGO2 is important in regulating T-cell functions, Amigo2-overexpression dampens NF-kB transcriptional activity, while Amigo2-deficiency enhances Akt but suppresses GSK-3β phosphorylation and promotes nuclear translocations of NF-kB and NFAT1 in Th-cells[1].

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q86SJ2 (V40-A392)

Gene ID

347902  [NCBI]

Synonyms
rHuAmphoterin-Induced Protein 2, His; AMIGO2; Alivin-1; Amphoterin-Induced Protein 2
AA Sequence

VCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHAHHHHHHH

Molecular Weight

58-85 kDa

Appearance

Lyophilized powder.

Formulation

Lyophilized after extensive dialysis against PB, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
References

AMIGO2 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AMIGO2 Protein, Human (HEK293, His)
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