AMIGO2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
AMIGO2 Protein, Human (HEK293, His) is an 58-85 kDa AMIGO2 protein fused His-tag expressed in HEK 293 cells. AMIGO2 Protein, Human (HEK293, His) interacts with PDK1 to activate the Akt pathway localizated to the plasma membrane[1].
Background
AMIGO2 is a scaffold protein that regulates PDK1 membrane localization and Akt activation. This protein contains six leucine-rich repeats (LRRs), a single IgG-like domain, a transmembrane domain, and a cytosolic domain. AMIGO2 plays its role by controling PDK1 localization to the plasma membrane and then directly interacts with PDK1 to activate the Akt pathway[1]. Loss of AMIGO2 in endothelial cells (ECs) leads to apoptosis and inhibition of angiogenesis with Akt inactivation[1]. AMIGO2 is important in regulating T-cell functions, Amigo2-overexpression dampens NF-kB transcriptional activity, while Amigo2-deficiency enhances Akt but suppresses GSK-3β phosphorylation and promotes nuclear translocations of NF-kB and NFAT1 in Th-cells[1].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q86SJ2 (V40-A392)
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Molecular Construction
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N-term
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AMIGO2 (V40-A392)
Accession # Q86SJ2 -
6*His
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
AMIGO2; Differentially Expressed In Gastric Adenocarcinoma; Adhesion Molecule With Ig Like Domain 2; Amphoterin-Induced Gene And Open Reading Frame 2; ALI1; Transmembrane Protein AMIGO2; DEGA; Amphoterin Induced Gene 2; Differentially Expressed In Gastric
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AA Sequence
VCPTACICATDIVSCTNKNLSKVPGNLFRLIKRLDLSYNRIGLLDSEWIPVSFAKLNTLILRHNNITSISTGSFSTTPNLKCLDLSSNKLKTVKNAVFQELKVLEVLLLYNNHISYLDPSAFGGLSQLQKLYLSGNFLTQFPMDLYVGRFKLAELMFLDVSYNRIPSMPMHHINLVPGKQLRGIYLHGNPFVCDCSLYSLLVFWYRRHFSSVMDFKNDYTCRLWSDSRHSRQVLLLQDSFMNCSDSIINGSFRALGFIHEAQVGERLMVHCDSKTGNANTDFIWVGPDNRLLEPDKEMENFYVFHNGSLVIESPRFEDAGVYSCIAMNKQRLLNETVDVTINVSNFTVSRSHA
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Predicted Molecular Mass
41.3 kDa
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Molecular Weight
Approximately 58-85 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PB, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (263 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Hyojin Park, et al.AMIGO2, a novel membrane anchor of PDK1, controls cell survival and angiogenesis via Akt activation. J Cell Biol [Content Brief]
[2]. Zhilin Li, et al. AMIGO2 modulates T cell functions and its deficiency in mice ameliorates experimental autoimmune encephalomyelitis. Brain Behav Immun. 2017 May;62:110-123. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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