ARMET/MANF Protein, Human (158a.a, HEK293, His)

ARMET/MANF Protein, Human (158a.a, HEK293, His) expresses in HEK293 cells with a His tag. Armet/MANF, localized in the ER and Golgi, is a secreted protein and acts as a gene up-regulated by various forms of ER stress^[1].

Armet (also known as MANF) acts as a gene up-regulated by various forms of ER stress in different cell lines and by cerebral ischaemia in rat. Armet interacts with mutant matrilin-3. Armet is a genotype-specific ER stress response protein with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in multiple epiphyseal dysplasia (MED) that could be exploited as a potential therapeutic target^[1].

Human

HEK293

C-6*His

P55145 (L25-L182)

7873  [NCBI]

LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDLHHHHHH

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB,150 mM NaCl, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Andria Apostolou, et al. Armet, a UPR-upregulated protein, inhibits cell proliferation and ER stress-induced cell death. Exp Cell Res. 2008 Aug 1;314(13):2454-67.  [Content Brief]