ARMET/MANF Protein, Human (HEK293, His)
Based on 1 Customer Validation
ARMET/MANF Protein, Human (HEK293, His) expresses in HEK293 cells with a His tag. Armet/MANF, localized in the ER and Golgi, is a secreted protein and acts as a gene up-regulated by various forms of ER stress.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
ARMET/MANF Protein, Human (HEK293, His) expresses in HEK293 cells with a His tag. Armet/MANF, localized in the ER and Golgi, is a secreted protein and acts as a gene up-regulated by various forms of ER stress[1].
Background
Armet (also known as MANF) acts as a gene up-regulated by various forms of ER stress in different cell lines and by cerebral ischaemia in rat. Armet interacts with mutant matrilin-3. Armet is a genotype-specific ER stress response protein with substrate specificities, and that aggregation of mutant matrilin-3 is a key disease trigger in multiple epiphyseal dysplasia (MED) that could be exploited as a potential therapeutic target[1].
Verified Bioactivity
Measured in a cell proliferation assay using rat C6 cells. The ED50 for this effect is 1.14 μg/mL, corresponding to a specific activity is 877.192 units/mg.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
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Bioactivity - Cell-Based Assay
Bioactivity - Cell-Based Assay
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P55145 (L25-L182)
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Molecular Construction
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N-term
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ARMET (L25-L182)
Accession # P55145 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
MANF; Arginine-Rich, Mutated In Early Stage Tumors; Prev. ARMET; Protein ARMET; ARP; DDDS; Mesencephalic Astrocyte-Derived Neurotrophic Factor; Mesencephalic Astrocyte Derived Neurotrophic Factor; Arginine-Rich Protein
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AA Sequence
LRPGDCEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREARGKENRLCYYIGATDDAATKIINEVSKPLAHHIPVEKICEKLKKKDSQICELKYDKQIDLSTVDLKKLRVKELKKILDDWGETCKGCAEKSDYIRKINELMPKYAPKAASARTDL
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Predicted Molecular Mass
19 kDa
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Molecular Weight
Approximately 17-21 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB,150 mM NaCl, pH7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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