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  4. BACE2 Protein, Mouse (HEK293, His)

BACE2 Protein, Mouse (HEK293, His)

Cat. No.: HY-P75588
COA Handling Instructions

The BACE2 protein is critically involved in the proteolytic processing of amyloid precursor protein (APP), producing beta-cleaved soluble APP and a cell-associated C-terminal fragment. It cleaves APP at residues 690-691 and 671-672. BACE2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived BACE2 protein, expressed by HEK293 , with C-His labeled tag. The total length of BACE2 Protein, Mouse (HEK293, His) is 443 a.a., with molecular weight of ~55 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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  • Documentation

Description

The BACE2 protein is critically involved in the proteolytic processing of amyloid precursor protein (APP), producing beta-cleaved soluble APP and a cell-associated C-terminal fragment. It cleaves APP at residues 690-691 and 671-672. BACE2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived BACE2 protein, expressed by HEK293 , with C-His labeled tag. The total length of BACE2 Protein, Mouse (HEK293, His) is 443 a.a., with molecular weight of ~55 kDa.

Background

The BACE2 protein assumes a critical role in the proteolytic processing of the amyloid precursor protein (APP), cleaving it between residues 690 and 691. This cleavage event results in the generation and extracellular release of beta-cleaved soluble APP and a corresponding cell-associated C-terminal fragment, later released by gamma-secretase. Additionally, BACE2 has been shown to cleave APP between residues 671 and 672. Beyond its involvement with APP, BACE2 plays a pivotal role in the proteolytic shedding of PMEL during early stages of melanosome biogenesis, cleaving PMEL within the M-beta fragment to release the amyloidogenic PMEL luminal fragment comprising M-alpha and a small portion of M-beta N-terminus. This cleavage step is crucial for the subsequent processing and assembly of PMEL fibrils into amyloid sheets. Furthermore, BACE2 is responsible for the proteolytic processing of CLTRN in pancreatic beta cells, highlighting its diverse functions in cellular processes and contributing to our understanding of its significance in various physiological contexts.

Biological Activity

Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2. The specific activity is 2187.098 pmol/min/µg, as measured under the described conditions.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q9JL18 (A20-P462)

Gene ID
Molecular Construction
N-term
BACE2 (A20-P462)
Accession # Q9JL18
His
C-term
Synonyms
Beta-secretase 2; Aspartyl protease 1; ASP1; Memapsin-1; Bace2
AA Sequence

AVPALAPAPFTLPLQVAGATNHRASAVPGLGTPELPRADGLALALEPVRATANFLAMVDNLQGDSGRGYYLEMLIGTPPQKVQILVDTGSSNFAVAGAPHSYIDTYFDSESSSTYHSKGFDVTVKYTQGSWTGFVGEDLVTIPKGFNSSFLVNIATIFESENFFLPGIKWNGILGLAYAALAKPSSSLETFFDSLVAQAKIPDIFSMQMCGAGLPVAGSGTNGGSLVLGGIEPSLYKGDIWYTPIKEEWYYQIEILKLEIGGQNLNLDCREYNADKAIVDSGTTLLRLPQKVFDAVVEAVARTSLIPEFSDGFWTGAQLACWTNSETPWAYFPKISIYLRDENASRSFRITILPQLYIQPMMGAGFNYECYRFGISSSTNALVIGATVMEGFYVVFDRAQRRVGFAVSPCAEIEGTTVSEISGPFSTEDIASNCVPAQALNEP

Molecular Weight

Approximately 55 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

BACE2 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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BACE2 Protein, Mouse (HEK293, His)
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HY-P75588
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