Bcl-XL Protein, Mouse (His, solution)
Based on 1 Customer Validation
Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (His, solution) is the recombinant mouse-derived Bcl-XL protein, expressed by E. coli , with C-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, but its antiapoptotic activity does not require heterodimerization with BAX. Bcl-XL Protein, Mouse (His, solution) is the recombinant mouse-derived Bcl-XL protein, expressed by E. coli , with C-His labeled tag.
Background
Bcl-XL protein forms heterodimers with BAX, BAK, or BCL2, with heterodimerization with BAX not being essential for its anti-apoptotic activity. Additionally, it interacts with isoform 1 of SIVA1, inhibiting its anti-apoptotic function. The protein also engages with IKZF3 and RTL10/BOP. Furthermore, interactions with DNM1L and CLTA suggest the potential formation of a complex in synaptic vesicles that includes clathrin and MFF. Notably, Bcl-XL interacts with NLRP1, specifically via the loop between motifs BH4 and BH3, but does not engage with NLRP2, NLRP3, NLRP4, PYCARD, or MEFV. It also interacts with BECN1, indicating its involvement in autophagy regulation.
Verified Bioactivity
1. Immobilized human BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.
2. Immobilized mouse BID at 10 μg/mL (100 μl/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.
Technical Parameters
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Species Mouse
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Source E. coli
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Tag C-His
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Accession
Q64373-1 (M1-R212)
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Molecular Construction
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N-term
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Bcl-XL (M1-R212)
Accession # Q64373-1 -
His
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C-term
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Protein Length
Partial
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Synonyms
BCL2L1; Bcl-X; BCL2 Like 1; Protein Phosphatase 1, Regulatory Subunit 52; BCL2L; Bcl-XL; BCLX; Bcl-XS; Apoptosis Regulator Bcl-X; BCL-XL/S; Bcl-2-Like Protein 1; Bcl2-L-1; PPP1R52
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AA Sequence
MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEAERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNR
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Predicted Molecular Mass
25.2 kDa
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Molecular Weight
Approximately 33 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.5.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (235 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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