BNIP3L Protein, Human

BNIP3L, a pro-apoptotic member of the Bcl-2 family, possesses a BH3 domain and binds to Bcl-2. It directly targets mitochondria, inducing apoptotic changes like membrane potential loss and cytochrome c release. With ubiquitous expression, especially in fat and ovary, the gene suggests involvement in diverse physiological processes across multiple organs, emphasizing its potential significance. BNIP3L Protein, Human is the recombinant human-derived BNIP3L protein, expressed by E. coli , with tag free.

The BNIP3L Protein is a member of the pro-apoptotic subfamily within the Bcl-2 family, featuring a BH3 domain and the ability to bind to Bcl-2. This protein exerts its pro-apoptotic effects by directly targeting mitochondria, leading to apoptotic changes such as the loss of membrane potential and the release of cytochrome c. Its role in the regulation of apoptosis suggests its involvement in crucial cellular processes. The gene displaying ubiquitous expression, with prominent levels in fat (RPKM 69.4), ovary (RPKM 49.9), and 24 other tissues, underscores its potential significance in diverse physiological contexts across various organs.

Human

E. coli

Tag Free

NP_004322.1 (S2-K187)

665  [NCBI]

Partial

SSHLVEPPPPLHNNNNNCEENEQSLPPPAGLNSSWVELPMNSSNGNDNGNGKNGGLEHVPSSSSIHNGDMEKILLDAQHESGQSSSRGSSHCDSPSPQEDGQIMFDVEMHTSRDHSSQSEEEVVEGEKEVEALKKSADWVSDWSSRPENIPPKEFHFRHPKRSVSLSMRKSGAMKKGGIFSAEFLK

Approximately 32-36 kDa

• 
  ≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris-HCl, 300 mM NaCl, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.