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C-MPL Protein, Human (HEK293, His)

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C-MPL Protein, the thrombopoietin receptor, is a pivotal regulator of megakaryopoiesis and platelet production, suggesting a specific role in TPO-R-dependent immune responses. Forming homodimers and interacting with ATXN2L, it contributes to its multifaceted role in hematopoiesis and immune modulation. C-MPL Protein, Human (HEK293, His) is the recombinant human-derived C-MPL protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • C-MPL Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

C-MPL Protein, the thrombopoietin receptor, is a pivotal regulator of megakaryopoiesis and platelet production, suggesting a specific role in TPO-R-dependent immune responses. Forming homodimers and interacting with ATXN2L, it contributes to its multifaceted role in hematopoiesis and immune modulation. C-MPL Protein, Human (HEK293, His) is the recombinant human-derived C-MPL protein, expressed by HEK293 , with C-His labeled tag.

Background

C-MPL Protein serves as the receptor for thrombopoietin, playing a pivotal role as a primary regulator of megakaryopoiesis and platelet production. Its involvement suggests a potential regulatory function specific to TPO-R-dependent immune responses. The protein forms homodimers and interacts with ATXN2L, contributing to its multifaceted role in hematopoiesis and immune modulation.

Verified Bioactivity

1.Measured by its binding ability in a functional ELISA. When immobilized Human c-MPL, His Tag at 0.2 μg/mL, can bind Anti-c-MPL Antibody, hFc Tag with the EC50 of 14.3 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human c-MPL, His Tag at 0.5 μg/mL (100 μL/Well) on the plate. Dose response curve for Anti-c-MPL Antibody, hFc Tag with the EC50 of 1.0-10.0 ng/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-8*His
  • Accession

    P40238-1 (Q26-W491)

  • Gene ID
    4352  [NCBI]
  • Molecular Construction
    • N-term
    • C-MPL (Q26-W491)
      Accession # P40238-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MPL; Myeloproliferative Leukemia Virus Oncogene; MPL Proto-Oncogene, Thrombopoietin Receptor; TPO-R; TPOR; Thrombopoietin Receptor Variant; Myeloproliferative Leukemia Protein; CD110 Antigen; Thrombopoietin Receptor; THCYT2; Proto-Oncogene C-Mpl; C-MPL; C

  • AA Sequence

    QDVSLLASDSEPLKCFSRTFEDLTCFWDEEEAAPSGTYQLLYAYPREKPRACPLSSQSMPHFGTRYVCQFPDQEEVRLFFPLHLWVKNVFLNQTRTQRVLFVDSVGLPAPPSIIKAMGGSQPGELQISWEEPAPEISDFLRYELRYGPRDPKNSTGPTVIQLIATETCCPALQRPHSASALDQSPCAQPTMPWQDGPKQTSPSREASALTAEGGSCLISGLQPGNSYWLQLRSEPDGISLGGSWGSWSLPVTVDLPGDAVALGLQCFTLDLKNVTCQWQQQDHASSQGFFYHSRARCCPRDRYPIWENCEEEEKTNPGLQTPQFSRCHFKSRNDSIIHILVEVTTAPGTVHSYLGSPFWIHQAVRLPTPNLHWREISSGHLELEWQHPSSWAAQETCYQLRYTGEGHQDWKVLEPPLGARGGTLELRPRSRYRLQLRARLNGPTYQGPWSSWSDPTRVETATETAW

  • Predicted Molecular Mass

    53.6 kDa

  • Molecular Weight

    Approximately 60-77 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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