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CADM4/IGSF4C Protein, Human (HEK293, His)

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Based on 1 Customer Validation

CADM4/IGSF4C Protein is crucial for calcium- and magnesium-independent cell-cell adhesion, actively mediating cellular interactions. Apart from its adhesive role, it is speculated to act as a tumor suppressor, hinting at a potential regulatory role in cell growth and tumorigenic processes. The dual attributes of mediating adhesion and potentially suppressing tumors highlight the multifaceted nature of CADM4/IGSF4C in cellular processes. CADM4/IGSF4C Protein, Human (HEK293, His) is the recombinant human-derived CADM4/IGSF4C protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CADM4/IGSF4C Protein, Human (HEK293, His)
  • Experimental Validation Results for CADM4/IGSF4C Protein, Human (HEK293, His)
  • CADM4/IGSF4C Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

CADM4/IGSF4C Protein is crucial for calcium- and magnesium-independent cell-cell adhesion, actively mediating cellular interactions. Apart from its adhesive role, it is speculated to act as a tumor suppressor, hinting at a potential regulatory role in cell growth and tumorigenic processes. The dual attributes of mediating adhesion and potentially suppressing tumors highlight the multifaceted nature of CADM4/IGSF4C in cellular processes. CADM4/IGSF4C Protein, Human (HEK293, His) is the recombinant human-derived CADM4/IGSF4C protein, expressed by HEK293 , with C-His labeled tag.

Background

The CADM4/IGSF4C Protein plays a crucial role in cell-cell adhesion, demonstrating calcium- and magnesium-independent adhesion activity. This protein, with its monomeric and homodimeric forms, actively participates in mediating cellular interactions. Beyond its adhesive functions, CADM4/IGSF4C is speculated to possess tumor-suppressor activity, suggesting a potential role in regulating cell growth and suppressing tumorigenic processes. The dual attributes of mediating cell-cell adhesion and potentially exerting tumor-suppressive effects underscore the multifaceted nature of CADM4/IGSF4C in cellular processes.

Verified Bioactivity

Measured by its ability to inhibit the proliferation of A549 cells. The ED50 for this effect is 0.4653 μg/mL, corresponding to a specific activity is 2.149×103 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the proliferation of A549 cells. The ED50 for this effect is 0.4653 μg/mL, corresponding to a specific activity is 2.149×103 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q8NFZ8 (P21-Y323)

  • Gene ID
    199731  [NCBI]
  • Molecular Construction
    • N-term
    • CADM4 (P21-Y323)
      Accession # Q8NFZ8
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    CADM4; TSLC1-Like Protein 2; Prev. IGSF4C; NECL4; TSLL2; Immunoglobulin Superfamily, Member 4C; SynCAM4; TSLC1-Like 2; Necl-4; NECL-4; Immunoglobulin Superfamily Member 4C; IgSF4C; Nectin-Like Protein 4; Cell Adhesion Molecule 4; Nectin-Like 4

  • AA Sequence

    PGAGQEVQTENVTVAEGGVAEITCRLHQYDGSIVVIQNPARQTLFFNGTRALKDERFQLEEFSPRRVRIRLSDARLEDEGGYFCQLYTEDTHHQIATLTVLVAPENPVVEVREQAVEGGEVELSCLVPRSRPAATLRWYRDRKELKGVSSSQENGKVWSVASTVRFRVDRKDDGGIIICEAQNQALPSGHSKQTQYVLDVQYSPTARIHASQAVVREGDTLVLTCAVTGNPRPNQIRWNRGNESLPERAEAVGETLTLPGLVSADNGTYTCEASNKHGHARALYVLVVYDPGAVVEAQTSVPY

  • Molecular Weight

    Approximately 48-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the proliferation of A549 cells. The ED50 for this effect is 0.4653 μg/mL, corresponding to a specific activity is 2.149×103 units/mg.

  • CADM4/IGSF4C Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CADM4/IGSF4C Protein, Human (HEK293, His)
  • Experimental Validation Results for CADM4/IGSF4C Protein, Human (HEK293, His)
  • CADM4/IGSF4C Protein, Human (HEK293, His) Product Image