CCND1 Protein, Human (His)

The CCND1 protein is a regulatory component of the cyclin D1-CDK4 complex that coordinates phosphorylation and RB family inhibition to regulate the G(1)/S transition. This promotes the dissociation of E2F from the RB/E2F complex and promotes the transcription of E2F target genes critical for G(1) phase progression. CCND1 Protein, Human (His) is the recombinant human-derived CCND1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CCND1 Protein, Human (His) is 295 a.a., with molecular weight of ~37.7 kDa.

The CCND1 protein functions as a regulatory component of the cyclin D1-CDK4 (DC) complex, orchestrating phosphorylation and inhibition of retinoblastoma (RB) family members, including RB1, to modulate cell-cycle progression during G(1)/S transition. This phosphorylation event prompts the dissociation of the transcription factor E2F from the RB/E2F complex, facilitating the transcription of E2F target genes essential for G(1) phase progression. CCND1 also exhibits hypophosphorylation activity on RB1 in early G(1) phase. Serving as a major integrator of mitogenic and antimitogenic signals, cyclin D-CDK4 complexes, including CCND1, play a pivotal role in cell-cycle regulation. Additionally, CCND1 engages in interactions with various proteins, such as SMAD3, forming a ternary complex with CDK4 and CDKN1B, and displaying transcriptional corepressor activity with INSM1. These intricate interactions contribute to the nuanced regulatory functions of CCND1 in cellular processes.

Human

E. coli

N-6*His

P24385 (M1-I295)

595  [NCBI]

MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Approximately 37.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyopilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.