CCND1 Protein, Human (His)
Based on 1 Customer Validation
The CCND1 protein is a regulatory component of the cyclin D1-CDK4 complex that coordinates phosphorylation and RB family inhibition to regulate the G(1)/S transition. This promotes the dissociation of E2F from the RB/E2F complex and promotes the transcription of E2F target genes critical for G(1) phase progression. CCND1 Protein, Human (His) is the recombinant human-derived CCND1 protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CCND1 protein is a regulatory component of the cyclin D1-CDK4 complex that coordinates phosphorylation and RB family inhibition to regulate the G(1)/S transition. This promotes the dissociation of E2F from the RB/E2F complex and promotes the transcription of E2F target genes critical for G(1) phase progression. CCND1 Protein, Human (His) is the recombinant human-derived CCND1 protein, expressed by E. coli , with N-6*His labeled tag.
Background
The CCND1 protein functions as a regulatory component of the cyclin D1-CDK4 (DC) complex, orchestrating phosphorylation and inhibition of retinoblastoma (RB) family members, including RB1, to modulate cell-cycle progression during G(1)/S transition. This phosphorylation event prompts the dissociation of the transcription factor E2F from the RB/E2F complex, facilitating the transcription of E2F target genes essential for G(1) phase progression. CCND1 also exhibits hypophosphorylation activity on RB1 in early G(1) phase. Serving as a major integrator of mitogenic and antimitogenic signals, cyclin D-CDK4 complexes, including CCND1, play a pivotal role in cell-cycle regulation. Additionally, CCND1 engages in interactions with various proteins, such as SMAD3, forming a ternary complex with CDK4 and CDKN1B, and displaying transcriptional corepressor activity with INSM1. These intricate interactions contribute to the nuanced regulatory functions of CCND1 in cellular processes.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
P24385 (M1-I295)
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Molecular Construction
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N-term
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6*His
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CCND1 (M1-I295)
Accession # P24385 -
C-term
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Protein Length
Full Length
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Synonyms
CCND1; B-Cell CLL/Lymphoma 1; Prev. PRAD1; PRAD1 Oncogene; Prev. BCL1; Parathyroid Adenomatosis 1; Prev. D11S287E; Cyclin D1, Isoform CRA_c; U21B31; G1/S-Specific Cyclin D1; Cyclin D1 (PRAD1: Parathyroid Adenomatosis 1); CCND1 Protein; B-Cell Lymphoma 1 P
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AA Sequence
MEHQLLCCEVETIRRAYPDANLLNDRVLRAMLKAEETCAPSVSYFKCVQKEVLPSMRKIVATWMLEVCEEQKCEEEVFPLAMNYLDRFLSLEPVKKSRLQLLGATCMFVASKMKETIPLTAEKLCIYTDNSIRPEELLQMELLLVNKLKWNLAAMTPHDFIEHFLSKMPEAEENKQIIRKHAQTFVALCATDVKFISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI
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Predicted Molecular Mass
43.4 kDa
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Molecular Weight
Approximately 37-42 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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