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CD146/MCAM Protein, Human (HEK293, His)

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The CD146/MCAM protein is critical for maintaining endothelial monolayer integrity and cell adhesion in vascular tissue. Its expression promotes the interaction of melanoma cells with the vasculature and may contribute to tumor spread through the bloodstream. CD146/MCAM Protein, Human (HEK293, His) is the recombinant human-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • CD146/MCAM Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The CD146/MCAM protein is critical for maintaining endothelial monolayer integrity and cell adhesion in vascular tissue. Its expression promotes the interaction of melanoma cells with the vasculature and may contribute to tumor spread through the bloodstream. CD146/MCAM Protein, Human (HEK293, His) is the recombinant human-derived CD146/MCAM protein, expressed by HEK293 , with C-His labeled tag.

Background

The CD146/MCAM protein plays a crucial role in cell adhesion and maintaining the integrity of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression enables melanoma cells to interact with the cellular components of the vascular system, potentially promoting the spread of tumors through the bloodstream. During embryonic development, CD146/MCAM may function as an adhesion molecule in neural crest cells. It acts as a surface receptor, initiating the tyrosine phosphorylation of FYN and PTK2/FAK1, and leading to a transient rise in intracellular calcium levels.

Verified Bioactivity

Immobilized Human MUC18, His Tag at 1 μg/mL (100 μL/well) on the plate. Dose response curve for Anti-MUC18, hFc Tag with the EC50 of 4-5 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    P43121-1 (V24-G559)

  • Gene ID
    4162  [NCBI]
  • Molecular Construction
    • N-term
    • MCAM (V24-G559)
      Accession # P43121-1
    • His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    MCAM; Melanoma-Associated Antigen MUC18; Melanoma Cell Adhesion Molecule; Cell Surface Glycoprotein MUC18; MUC18; Cell Surface Glycoprotein P1H12; MelCAM; Melanoma-Associated Antigen A32; METCAM; Melanoma Adhesion Molecule; HEMCAM; Gicerin; CD146; CD146 A

  • AA Sequence

    VPGEAEQPAPELVEVEVGSTALLKCGLSQSQGNLSHVDWFSVHKEKRTLIFRVRQGQGQSEPGEYEQRLSLQDRGATLALTQVTPQDERIFLCQGKRPRSQEYRIQLRVYKAPEEPNIQVNPLGIPVNSKEPEEVATCVGRNGYPIPQVIWYKNGRPLKEEKNRVHIQSSQTVESSGLYTLQSILKAQLVKEDKDAQFYCELNYRLPSGNHMKESREVTVPVFYPTEKVWLEVEPVGMLKEGDRVEIRCLADGNPPPHFSISKQNPSTREAEEETTNDNGVLVLEPARKEHSGRYECQGLDLDTMISLLSEPQELLVNYVSDVRVSPAAPERQEGSSLTLTCEAESSQDLEFQWLREETGQVLERGPVLQLHDLKREAGGGYRCVASVPSIPGLNRTQLVNVAIFGPPWMAFKERKVWVKENMVLNLSCEASGHPRPTISWNVNGTASEQDQDPQRVLSTLNVLVTPELLETGVECTASNDLGKNTSILFLELVNLTTLTPDSNTTTGLSTSTASPHTRANSTSTERKLPEPESRG

  • Predicted Molecular Mass

    61.3 kDa

  • Molecular Weight

    Approximately 70-100 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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