CDK1 Protein, Human (sf9, GST)

The CDK1/CDC2-cyclin-B complex is a key regulator in the cell cycle. It plays an important role in cell division and development and regulates a variety of cellular activities, including chromosome segregation, nuclear membrane rupture, cytokinesis, etc. The activity of CDK1 is regulated by a variety of phosphorylation and dephosphorylation, thereby controlling the progression of the cell cycle and the DNA repair process. CDK1 Protein, Human (sf9, GST) is the recombinant human-derived CDK1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CDK1 Protein, Human (sf9, GST) is 297 a.a., with molecular weight of ~53 kDa.

The CDK1/CDC2-cyclin-B complex is a key regulator of the cell cycle. It plays an important control role in the interphase fertilized egg and is crucial for the early stages of embryonic development. During G2 and early mitosis, CDC25A/B/C-mediated dephosphorylation activates the CDK1/cyclin complex. This complex phosphorylates multiple substrates, triggering centrosome dissociation, Golgi dynamics, nuclear envelope disruption, and chromosome condensation. After chromosomes are condensed and aligned on the metaphase plate, CDK1 activity is turned off by WEE1- and PKMYT1-mediated phosphorylation, allowing sister chromatid separation, chromosome decondensation, nuclear envelope remodeling, and cytokinesis. When DNA is damaged, CDK1 will be inactivated by phosphorylation mediated by PKR/EIF2AK2 and WEE1, thereby stopping the cell cycle and genome replication and promoting DNA repair. After repair is completed, CDK1 is reactivated by WIP1-dependent signaling to resume cell cycle progression. In proliferating cells, CDK1-mediated phosphorylation can inhibit FOXO1 interactions and promote FOXO1 nuclear accumulation and transcription factor activity, leading to cell death of postmitotic neurons. In addition, phosphorylation of CDK1 can also regulate microtubule dynamics, spindle formation and other processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

P06493 (M1-M297)

983  [NCBI]

Approximately 53 kDa

Greater than 84% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, 0.5 mM GSH, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.