CK2 alpha/CSNK2A1 Protein, Human (sf9, GST)

CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CK2 alpha/CSNK2A1 Protein, Human (sf9, GST) is the recombinant human-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of CK2 alpha/CSNK2A1 Protein, Human (sf9, GST) is 391 a.a., with molecular weight of ~65 KDa.

The catalytic subunit of CSNK2A2 protein functions as a core component of a constitutively active serine/threonine-protein kinase complex, showcasing a broad substrate specificity for acidic residues C-terminal to the phosphorylated serine or threonine. Operating as a regulatory node, CSNK2A2 plays a pivotal role in diverse cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. It contributes to the p53/TP53-dependent spindle assembly checkpoint during mitosis, maintaining cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Additionally, CSNK2A2 is implicated in apoptotic regulation, phosphorylating key players like caspases and NOL3. Its influence extends to transcriptional regulation, with direct phosphorylation of RNA polymerases and numerous transcription factors. In the intricate landscape of cellular signaling, CSNK2A2 participates in Wnt signaling, acts as an ectokinase for extracellular proteins, and phosphorylates various proteins involved in the viral life cycles of diverse viruses. Moreover, it plays a crucial role in the circadian clock function and contributes to the formation of a membraneless compartment by phosphorylating FMR1. Overall, CSNK2A2 emerges as a central orchestrator of cellular responses to diverse stimuli, showcasing its significance in maintaining cellular homeostasis.

The specific activity was determined to be 38 nmol/min/mg using casein as substrate.

Human

Sf9 insect cells

N-GST

P68400-1 (M1-Q391)

1457  [NCBI]

Approximately 65 kDa.

Greater than 90% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.