1. Recombinant Proteins
  2. Enzymes & Regulators
  3. CRISPR-Cas9 Protein, S. pyogenes

CRISPR-Cas9 Protein, S. pyogenes

Cat. No.: HY-P77727
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Stock
100 μg Ask For Quote & Lead Time

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CRISPR-Cas9 Protein, S. pyogenes Related Classifications

  • Technical Parameters

  • Properties

  • Documentation

Species

Others

Source

E.coli

Accession

Q99ZW2 (D2-D1368)

Gene ID

/

Synonyms
CRISPR-associated endonuclease Cas9/Csn1; cas9; SpCas9; SpyCas9
AA Sequence

Molecular Weight

Approximately 163 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC

Biological Activity
Data is not available.
Appearance

Solution.

Formulation

Supplied as 0.22 μm filtered solution in 30 mM Tris-HCl, 0.3 M NaCl, 50% glycerol, 0.1 mM EDTA, pH 7.4.

Endotoxin Level

<0.01 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CRISPR-Cas9 Protein, S. pyogenes
Cat. No.:
HY-P77727
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