Cyclin E Protein, Human (SF9, His-GST)

Cyclin E controls the G1/S transition of the cell cycle. Cyclin E Protein, Human (SF9, His-GST) is the recombinant human-derived Cyclin E protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Cyclin E Protein, Human (SF9, His-GST) is 410 a.a., with molecular weight of ~70 kDa.

Cyclin E, an integral protein in the intricate regulation of the cell cycle, plays a pivotal role in governing the transition from the G1 phase to the S phase. Through its interaction with the CDK2 protein kinase, Cyclin E forms a dynamic serine/threonine kinase holoenzyme complex, with the cyclin subunit conferring substrate specificity to this crucial enzymatic assembly. Cyclin E is part of various complexes, including those involving CDK2, CABLES1, and CCNA1, as well as a complex comprising UHRF2, CDK2, and CCNE1. The interaction with UHRF2 leads to the ubiquitination of Cyclin E independently of its phosphorylation status, showcasing the intricate regulatory mechanisms at play. Additionally, Cyclin E engages in interactions with INCA1, further contributing to its multifaceted involvement in cell cycle control.

Human

Sf9 insect cells

N-His;N-GST

P24864 (M1-A410)

898  [NCBI]

MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA

Approximately 70 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.