Cyclin E Protein, Human (sf9, His-GST)
Cyclin E controls the G1/S transition of the cell cycle. Cyclin E Protein, Human (SF9, His-GST) is the recombinant human-derived Cyclin E protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Cyclin E controls the G1/S transition of the cell cycle. Cyclin E Protein, Human (SF9, His-GST) is the recombinant human-derived Cyclin E protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
Background
Cyclin E, an integral protein in the intricate regulation of the cell cycle, plays a pivotal role in governing the transition from the G1 phase to the S phase. Through its interaction with the CDK2 protein kinase, Cyclin E forms a dynamic serine/threonine kinase holoenzyme complex, with the cyclin subunit conferring substrate specificity to this crucial enzymatic assembly. Cyclin E is part of various complexes, including those involving CDK2, CABLES1, and CCNA1, as well as a complex comprising UHRF2, CDK2, and CCNE1. The interaction with UHRF2 leads to the ubiquitination of Cyclin E independently of its phosphorylation status, showcasing the intricate regulatory mechanisms at play. Additionally, Cyclin E engages in interactions with INCA1, further contributing to its multifaceted involvement in cell cycle control.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-His;N-GST
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Accession
P24864 (M1-A410)
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Molecular Construction
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N-term
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His-GST
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Cyclin E (M1-A410)
Accession # P24864 -
C-term
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Protein Length
Full Length
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Synonyms
CCNE1; Cyclin E Variant Ex7del; Prev. CCNE; Cyclin Et; G1/S-Specific Cyclin-E1; PCCNE1; Cyclin E Variant Ex5del; Cyclin E1; Cyclin Es
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AA Sequence
MPRERRERDAKERDTMKEDGGAEFSARSRKRKANVTVFLQDPDEEMAKIDRTARDQCGSQPWDNNAVCADPCSLIPTPDKEDDDRVYPNSTCKPRIIAPSRGSPLPVLSWANREEVWKIMLNKEKTYLRDQHFLEQHPLLQPKMRAILLDWLMEVCEVYKLHRETFYLAQDFFDRYMATQENVVKTLLQLIGISSLFIAAKLEEIYPPKLHQFAYVTDGACSGDEILTMELMIMKALKWRLSPLTIVSWLNVYMQVAYLNDLHEVLLPQYPQQIFIQIAELLDLCVLDVDCLEFPYGILAASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSGKKQSSGPEMA
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Predicted Molecular Mass
75 kDa
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Molecular Weight
Approximately 70 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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