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Draxin Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The Draxin protein is critical in commissural development in the spinal cord and forebrain, serving as a chemical pulse guidance protein for commissural axons. It inhibits dorsal spinal cord neurite outgrowth and acts as a Wnt signaling antagonist by interacting with LRP6, inhibiting cytosolic β-catenin stabilization. Draxin Protein, Human (HEK293, His) is the recombinant human-derived Draxin protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Draxin Protein, Human (HEK293, His)
  • Experimental Validation Results for Draxin Protein, Human (HEK293, His)
  • Draxin Protein, Human (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The Draxin protein is critical in commissural development in the spinal cord and forebrain, serving as a chemical pulse guidance protein for commissural axons. It inhibits dorsal spinal cord neurite outgrowth and acts as a Wnt signaling antagonist by interacting with LRP6, inhibiting cytosolic β-catenin stabilization. Draxin Protein, Human (HEK293, His) is the recombinant human-derived Draxin protein, expressed by HEK293 , with C-His labeled tag.

Background

Draxin protein plays a crucial role in the development of spinal cord and forebrain commissures, acting as a chemorepulsive guidance protein for commissural axons. Its function extends to inhibiting or repelling neurite outgrowth from the dorsal spinal cord. Additionally, Draxin serves as an antagonist of the Wnt signaling pathway by inhibiting the stabilization of cytosolic beta-catenin (CTNNB1) through its interaction with LRP6. This multifaceted functionality underscores the significance of Draxin in guiding axonal development and modulating key signaling pathways during neural development, highlighting its intricate involvement in shaping the intricate architecture of the nervous system.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized Human Draxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human LRP6 (HY-P77990). The KD is 7.243 nM.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Human Draxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human LRP6 (HY-P77990). The KD is 7.243 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q8NBI3 (G26-V349)

  • Gene ID
    374946  [NCBI]
  • Molecular Construction
    • N-term
    • Draxin (G26-V349)
      Accession # Q8NBI3
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    DRAXIN; FLJ34999; Prev. C1orf187; Chromosome 1 Open Reading Frame 187; Neucrin; AGPA3119; Dorsal Repulsive Axon Guidance Protein; UNQ3119; Neural Tissue-Specific Cysteine-Rich Protein; Dorsal Inhibitory Axon Guidance Protein; Draxin

  • AA Sequence

    GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV

  • Molecular Weight

    Approximately 52-58 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Human Draxin at 2 μg/mL (100 μL/well) can bind Biotinylated Human LRP6 (HY-P77990). The KD is 7.243 nM.

  • Draxin Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Draxin Protein, Human (HEK293, His)
  • Experimental Validation Results for Draxin Protein, Human (HEK293, His)
  • Draxin Protein, Human (HEK293, His) Product Image