Draxin Protein, Human (HEK293, His)

The Draxin protein is critical in commissural development in the spinal cord and forebrain, serving as a chemical pulse guidance protein for commissural axons. It inhibits dorsal spinal cord neurite outgrowth and acts as a Wnt signaling antagonist by interacting with LRP6, inhibiting cytosolic β-catenin stabilization. Draxin Protein, Human (HEK293, His) is the recombinant human-derived Draxin protein, expressed by HEK293 , with C-His labeled tag. The total length of Draxin Protein, Human (HEK293, His) is 349 a.a., with molecular weight of 50-60 kDa.

Draxin protein plays a crucial role in the development of spinal cord and forebrain commissures, acting as a chemorepulsive guidance protein for commissural axons. Its function extends to inhibiting or repelling neurite outgrowth from the dorsal spinal cord. Additionally, Draxin serves as an antagonist of the Wnt signaling pathway by inhibiting the stabilization of cytosolic beta-catenin (CTNNB1) through its interaction with LRP6. This multifaceted functionality underscores the significance of Draxin in guiding axonal development and modulating key signaling pathways during neural development, highlighting its intricate involvement in shaping the intricate architecture of the nervous system.

Human

HEK293

C-His

Q8NBI3 (G26-V349)

374946  [NCBI]

GALAPGTPARNLPENHIDLPGPALWTPQASHHRRRGPGKKEWGPGLPSQAQDGAVVTATRQASRLPEAEGLLPEQSPAGLLQDKDLLLGLALPYPEKENRPPGWERTRKRSREHKRRRDRLRLHQGRALVRGPSSLMKKAELSEAQVLDAAMEESSTSLAPTMFFLTTFEAAPATEESLILPVTSLRPQQAQPRSDGEVMPTLDMALFDWTDYEDLKPDGWPSAKKKEKHRGKLSSDGNETSPAEGEPCDHHQDCLPGTCCDLREHLCTPHNRGLNNKCFDDCMCVEGLRCYAKFHRNRRVTRRKGRCVEPETANGDQGSFINV

Approximately 50-60 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.