LRP-6 Protein, Human (HEK293, mFc)
Based on 1 Customer Validation
LRP-6 protein is a key component of the Wnt-Fzd-LRP5-LRP6 complex, a component of β-catenin signaling, and is essential for bone formation. LRP-6 acts as a cell surface co-receptor and coordinates the formation of signalosomes by clustering receptor-ligand complexes. LRP-6 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-6 protein, expressed by HEK293 , with C-mFc labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
LRP-6 protein is a key component of the Wnt-Fzd-LRP5-LRP6 complex, a component of β-catenin signaling, and is essential for bone formation. LRP-6 acts as a cell surface co-receptor and coordinates the formation of signalosomes by clustering receptor-ligand complexes. LRP-6 Protein, Human (HEK293, mFc) is the recombinant human-derived LRP-6 protein, expressed by HEK293 , with C-mFc labeled tag.
Background
As an integral component of the Wnt-Fzd-LRP5-LRP6 complex, LRP-6 protein plays a crucial role in initiating beta-catenin signaling by orchestrating the aggregation of receptor-ligand complexes into ribosome-sized signalosomes. Functioning as a cell-surface coreceptor in Wnt/beta-catenin signaling, it is particularly pivotal in bone formation. The complex formed by Wnt-induced Fzd/LRP6 coreceptor recruits DVL1 polymers to the plasma membrane, facilitating the recruitment of the AXIN1/GSK3B complex to the cell surface. This, in turn, promotes the formation of signalosomes, inhibiting AXIN1/GSK3-mediated phosphorylation and destruction of beta-catenin. LRP-6 is indispensable for the posterior patterning of the epiblast during gastrulation. The protein engages in a myriad of interactions, including those with various WNT proteins, FZD5, DKK1, DRAXIN, AXIN1, GRB10, RSPO1, RSPO3, SOST, MESD, CSNKIE, MACF1, DAB2, TMEM198, CAPRIN2, LYPD6, KREM1, and MDK, highlighting its versatility and regulatory roles in the Wnt signaling pathway.
Verified Bioactivity
1.Immobilized Human LRP-6, mFc Tag at 5 μg/mL (100 μl/Well) on plate. Dose response curve for Biotinylated Human SOST, His Tag with the EC50 of <2.1 μg/mL determined by ELISA.
2.Immobilized Biotinylated Human SOST, His Tag at 5 μg/mL (100μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human LRP-6, mFc Tag with the EC50 of ≤0.17 μg/mL determined by ELISA.
3.Immobilized Mouse Dkk-1, His Tag at 5 μg/mL (100 μL/well) can bind Human LRP-6 (20-630), Mouse IgG2a Fc Tag with the EC50 of 10-2300 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-mouse IgG2a Fc
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Accession
O75581 (A20-P630)
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Molecular Construction
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N-term
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LRP-6 (A20-P630)
Accession # O75581 -
mFc
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C-term
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Protein Length
Partial Extracellular Domain
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Synonyms
LRP6; Low Density Lipoprotein Receptor-Related Protein 6; LDL Receptor Related Protein 6; Low-Density Lipoprotein Receptor-Related Protein; Low-Density Lipoprotein Receptor-Related Protein 6; STHAG7; ADCAD2; OPTA4; LRP-6; EVR8
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AA Sequence
APLLLYANRRDLRLVDATNGKENATIVVGGLEDAAAVDFVFSHGLIYWSDVSEEAIKRTEFNKTESVQNVVVSGLLSPDGLACDWLGEKLYWTDSETNRIEVSNLDGSLRKVLFWQELDQPRAIALDPSSGFMYWTDWGEVPKIERAGMDGSSRFIIINSEIYWPNGLTLDYEEQKLYWADAKLNFIHKSNLDGTNRQAVVKGSLPHPFALTLFEDILYWTDWSTHSILACNKYTGEGLREIHSDIFSPMDIHAFSQQRQPNATNPCGIDNGGCSHLCLMSPVKPFYQCACPTGVKLLENGKTCKDGATELLLLARRTDLRRISLDTPDFTDIVLQLEDIRHAIAIDYDPVEGYIYWTDDEVRAIRRSFIDGSGSQFVVTAQIAHPDGIAVDWVARNLYWTDTGTDRIEVTRLNGTMRKILISEDLEEPRAIVLDPMVGYMYWTDWGEIPKIERAALDGSDRVVLVNTSLGWPNGLALDYDEGKIYWGDAKTDKIEVMNTDGTGRRVLVEDKIPHIFGFTLLGDYVYWTDWQRRSIERVHKRSAEREVIIDQLPDLMGLKATNVHRVIGSNPCAEENGGCSHLCLYRPQGLRCACPIGFELISDMKTCIVP
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Molecular Weight
Approximately 55 kDa & 110 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH 7.5, 10% trehalose.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (240 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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