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  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-1
  5. ENPP-1 Protein, Human (HEK293, His)

ENPP-1 Protein, Human (HEK293, His)

Cat. No.: HY-P77923
COA Handling Instructions

ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-1 Protein, Human (HEK293, His) is 828 a.a., with molecular weight of 110-120 kDa.

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Description

ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-1 Protein, Human (HEK293, His) is 828 a.a., with molecular weight of 110-120 kDa.

Background

ENPP-1 Protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. By preferentially hydrolyzing ATP and other nucleoside 5' triphosphates, such as GTP, CTP, and UTP, ENPP-1 generates diphosphate (PPi), which inhibits the further growth of nascent hydroxyapatite crystals, thereby preventing excessive bone mineralization and calcification of soft tissues. Additionally, it participates in the hydrolysis of diadenosine polyphosphates and 3',5'-cAMP to AMP, and may regulate nucleotide sugar availability in the endoplasmic reticulum and Golgi, influencing purinergic signaling. ENPP-1 also contributes to the repression of hedgehog signaling, inhibiting ectopic joint calcification and maintaining articular chondrocytes. Furthermore, it is implicated in modulating insulin sensitivity and function, participating in melanogenesis, and can hydrolyze 2',3'-cGAMP, a second messenger involved in triggering type-I interferon production. The exact role of 2',3'-cGAMP hydrolysis in the extracellular space remains unclear.

Biological Activity

Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is 117007.4074 pmol/min/μg, as measured under the described conditions.

Species

Human

Source

HEK293

Tag

C-His

Accession

P22413 (K98-D925)

Gene ID
Molecular Construction
N-term
ENPP-1 (K98-D925)
Accession # P22413
His
C-term
Synonyms
E-NPP 1; NPPase; ENPP1; NPPS; PC1; PDNP1; Ly-41 Antigen; M6S1; M6S1NPP1; NPP1; PCA1; PCA1ARHR2
AA Sequence

KPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRKEPVSDILKLKTHLPTFSQED

Molecular Weight

110-120 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ENPP-1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ENPP-1 Protein, Human (HEK293, His)
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HY-P77923
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