ENPP-1 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ENPP-1 protein is a nucleotide pyrophosphatase that inhibits excessive hydroxyapatite crystal growth by preferentially hydrolyzing ATP and other nucleoside triphosphates to generate PPi, thereby critically regulating bone mineralization and soft tissue calcification. ENPP-1 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-1 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
ENPP-1 Protein, a nucleotide pyrophosphatase, plays a crucial role in bone mineralization and soft tissue calcification by regulating pyrophosphate levels. By preferentially hydrolyzing ATP and other nucleoside 5' triphosphates, such as GTP, CTP, and UTP, ENPP-1 generates diphosphate (PPi), which inhibits the further growth of nascent hydroxyapatite crystals, thereby preventing excessive bone mineralization and calcification of soft tissues. Additionally, it participates in the hydrolysis of diadenosine polyphosphates and 3',5'-cAMP to AMP, and may regulate nucleotide sugar availability in the endoplasmic reticulum and Golgi, influencing purinergic signaling. ENPP-1 also contributes to the repression of hedgehog signaling, inhibiting ectopic joint calcification and maintaining articular chondrocytes. Furthermore, it is implicated in modulating insulin sensitivity and function, participating in melanogenesis, and can hydrolyze 2',3'-cGAMP, a second messenger involved in triggering type-I interferon production. The exact role of 2',3'-cGAMP hydrolysis in the extracellular space remains unclear.
Verified Bioactivity
1.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 35000 pmol/min/μg, as measured under the described conditions.
2.Immobilized Human ENPP-1, His Tag at 0.5 μg/mL (100 μl/well) on the plate. Dose response curve for Anti-ENPP-1 Antibody, hFc Tag with the EC50 of ≤20 ng/mL determined by ELISA.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
P22413 (K98-D925)
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Molecular Construction
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N-term
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ENPP-1 (K98-D925)
Accession # P22413 -
10*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
ENPP1; Nucleotide Diphosphatase; Prev. PDNP1; NPPase; Prev. M6S1; Membrane Component, Chromosome 6, Surface Marker 1; Prev. NPPS; Membrane Component Chromosome 6 Surface Marker 1; PC-1; Plasma-Cell Membrane Glycoprotein 1; PCA1; Alkaline Phosphodiesterase
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AA Sequence
KPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRKEPVSDILKLKTHLPTFSQED
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Predicted Molecular Mass
96.5 kDa
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Molecular Weight
Approximately 100-120 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (241 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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