ENPP-2 Protein, Human (HEK293, His)

The ENPP-2 protein catalyzes the hydrolysis of lysophospholipids (especially lysophosphatidylcholine) to produce the signaling molecule lysophosphatidic acid (LPA). It also acts on sphingosine phosphocholine to produce sphingosine 1-phosphate, which affects cell movement. ENPP-2 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-2 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ENPP-2 Protein, Human (HEK293, His) is 828 a.a., with molecular weight of 100-120 kDa.

The ENPP-2 Protein hydrolyzes lysophospholipids, specifically lysophosphatidylcholine, to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. It can also act on sphingosylphosphorylcholine, producing sphingosine-1-phosphate, which modulates cell motility. In addition, ENPP-2 Protein has been shown to hydrolyze bis-pNPP, pNP-TMP, and ATP to varying extents. It is involved in various motility-related processes, including angiogenesis and neurite outgrowth, and acts as an angiogenic factor by stimulating the migration of smooth muscle cells and microtubule formation. ENPP-2 Protein is also implicated in melanoma cell migration, potentially through a pertussis toxin-sensitive G protein. It may play a role in parturition induction, as well as in cell proliferation and adipose tissue development. Furthermore, it is required for LPA production in activated platelets and cleaves sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. ENPP-2 Protein exhibits a preference for the sn-1 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF).

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-6*His

AAH34961.1 (A36-I863)

5168  [NCBI]

AEGWEEGPPTVLSDSPWTNISGSCKGRCFELQEAGPPDCRCDNLCKSYTSCCHDFDELCLKTARGWECTKDRCGEVRNEENACHCSEDCLARGDCCTNYQVVCKGESHWVDDDCEEIKAAECPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHSPYMRPVYPTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDATFHLRGREKFNHRWWGGQPLWITATKQGVKAGTFFWSVVIPHERRILTILQWLTLPDHERPSVYAFYSEQPDFSGHKYGPFGPEMTNPLREIDKIVGQLMDGLKQLKLHRCVNVIFVGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRSKFSNNAKYDPKAIIANLTCKKPDQHFKPYLKQHLPKRLHYANNRRIEDIHLLVERRWHVARKPLDVYKKPSGKCFFQGDHGFDNKVNSMQTVFVGYGSTFKYKTKVPPFENIELYNVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPEEVTRPNYPGIMYLQSDFDLGCTCDDKVEPKNKLDELNKRLHTKGSTEERHLLYGRPAVLYRTRYDILYHTDFESGYSEIFLMPLWTSYTVSKQAEVSSVPDHLTSCVRPDVRVSPSFSQNCLAYKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWNYFQRVLVKKYASERNGVNVISGPIFDYDYDGLHDTEDKIKQYVEGSSIPVPTHYYSIITSCLDFTQPADKCDGPLSVSSFILPHRPDNEESCNSSEDESKWVEELMKMHTARVRDIEHLTSLDFFRKTSRSYPEILTLKTYLHTYESEI

100-120 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.