ENPP-7 Protein, Human (HEK293, His)

The ENPP-7 protein is a choline-specific phosphodiesterase that plays multiple roles in sphingomyelin digestion, ceramide formation, and fatty acid absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin, releases ceramide and phosphocholine, and promotes the breakdown of sphingomyelin. ENPP-7 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-7 Protein, Human (HEK293, His) is 418 a.a., with molecular weight of ~50-70 kDa.

ENPP-7 protein operates as a choline-specific phosphodiesterase with multifaceted roles in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption within the gastrointestinal tract. Notably, it hydrolyzes sphingomyelin, releasing ceramide and phosphocholine, thereby participating in the intricate processes of sphingomyelin breakdown and subsequent ceramide production. Additionally, ENPP-7 displays phospholipase C activity, cleaving phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF), leading to their inactivation. Despite lacking nucleotide pyrophosphatase activity, ENPP-7 may play a role in promoting cholesterol absorption by influencing the levels of sphingomyelin derived from both dietary and endogenous sources within the intestinal lumen. These diverse enzymatic activities highlight the multifunctional role of ENPP-7 in lipid metabolism and absorption processes, underscoring its significance in gastrointestinal physiology.

Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is measured by a Malachite Green Phosphate Detection Kit. The specific activity is 14449.85 pmoL/min/μg.

Human

HEK293

C-His

Q6UWV6 (A22-S439)

339221  [NCBI]

APVQSQGSQNKLLLVSFDGFRWNYDQDVDTPNLDAMARDGVKARYMTPAFVTMTSPCHFTLVTGKYIENHGVVHNMYYNTTSKVKLPYHATLGIQRWWDNGSVPIWITAQRQGLRAGSFFYPGGNVTYQGVAVTRSRKEGIAHNYKNETEWRANIDTVMAWFTEEDLDLVTLYFGEPDSTGHRYGPESPERREMVRQVDRTVGYLRESIARNHLTDRLNLIITSDHGMTTVDKRAGDLVEFHKFPNFTFRDIEFELLDYGPNGMLLPKEGRLEKVYDALKDAHPKLHVYKKEAFPEAFHYANNPRVTPLLMYSDLGYVIHGRINVQFNNGEHGFDNKDMDMKTIFRAVGPSFRAGLEVEPFESVHVYELMCRLLGIVPEANDGHLATLLPMLHTESALPPDGRPTLLPKGRSALPPSS

Approximately 50-70 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.