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ENPP-7 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

The ENPP-7 protein is a choline-specific phosphodiesterase that plays multiple roles in sphingomyelin digestion, ceramide formation, and fatty acid absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin, releases ceramide and phosphocholine, and promotes the breakdown of sphingomyelin. ENPP-7 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for ENPP-7 Protein, Human (HEK293, His)
  • ENPP-7 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The ENPP-7 protein is a choline-specific phosphodiesterase that plays multiple roles in sphingomyelin digestion, ceramide formation, and fatty acid absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin, releases ceramide and phosphocholine, and promotes the breakdown of sphingomyelin. ENPP-7 Protein, Human (HEK293, His) is the recombinant human-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag.

Background

ENPP-7 protein operates as a choline-specific phosphodiesterase with multifaceted roles in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption within the gastrointestinal tract. Notably, it hydrolyzes sphingomyelin, releasing ceramide and phosphocholine, thereby participating in the intricate processes of sphingomyelin breakdown and subsequent ceramide production. Additionally, ENPP-7 displays phospholipase C activity, cleaving phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF), leading to their inactivation. Despite lacking nucleotide pyrophosphatase activity, ENPP-7 may play a role in promoting cholesterol absorption by influencing the levels of sphingomyelin derived from both dietary and endogenous sources within the intestinal lumen. These diverse enzymatic activities highlight the multifunctional role of ENPP-7 in lipid metabolism and absorption processes, underscoring its significance in gastrointestinal physiology.

Verified Bioactivity

Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is measured by a Malachite Green Phosphate Detection Kit. The specific activity is 14449.85 pmoL/min/μg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q6UWV6 (A22-S439)

  • Gene ID
    339221  [NCBI]
  • Molecular Construction
    • N-term
    • ENPP-7 (A22-S439)
      Accession # Q6UWV6
    • His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    ENPP7; Alkaline Sphingomyelin Phosphodiesterase; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Intestinal Alkaline Sphingomyelinase; Alk-SMase; Alkaline Sphingomyelinase; NPP7; E-NPP 7; Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member

  • AA Sequence

    APVQSQGSQNKLLLVSFDGFRWNYDQDVDTPNLDAMARDGVKARYMTPAFVTMTSPCHFTLVTGKYIENHGVVHNMYYNTTSKVKLPYHATLGIQRWWDNGSVPIWITAQRQGLRAGSFFYPGGNVTYQGVAVTRSRKEGIAHNYKNETEWRANIDTVMAWFTEEDLDLVTLYFGEPDSTGHRYGPESPERREMVRQVDRTVGYLRESIARNHLTDRLNLIITSDHGMTTVDKRAGDLVEFHKFPNFTFRDIEFELLDYGPNGMLLPKEGRLEKVYDALKDAHPKLHVYKKEAFPEAFHYANNPRVTPLLMYSDLGYVIHGRINVQFNNGEHGFDNKDMDMKTIFRAVGPSFRAGLEVEPFESVHVYELMCRLLGIVPEANDGHLATLLPMLHTESALPPDGRPTLLPKGRSALPPSS

  • Molecular Weight

    Approximately 55-60 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • ENPP-7 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for ENPP-7 Protein, Human (HEK293, His)
  • ENPP-7 Protein, Human (HEK293, His) Product Image