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  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-7
  5. ENPP-7 Protein, Mouse (HEK293, His)

ENPP-7 Protein, Mouse (HEK293, His)

Cat. No.: HY-P76902
Handling Instructions

The ENPP-7 protein acts as a choline-specific phosphodiesterase and is essential for sphingomyelin digestion, ceramide formation, and FA absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin to release ceramide and phosphocholine. ENPP-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-7 Protein, Mouse (HEK293, His) is 400 a.a., with molecular weight of ~60 kDa.

For research use only. We do not sell to patients.

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Description

The ENPP-7 protein acts as a choline-specific phosphodiesterase and is essential for sphingomyelin digestion, ceramide formation, and FA absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin to release ceramide and phosphocholine. ENPP-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-7 Protein, Mouse (HEK293, His) is 400 a.a., with molecular weight of ~60 kDa.

Background

ENPP-7 protein functions as a choline-specific phosphodiesterase, playing a crucial role in sphingomyelin digestion, ceramide formation, and fatty acid (FA) absorption within the gastrointestinal tract. This enzyme hydrolyzes sphingomyelin, releasing ceramide and phosphocholine. Additionally, ENPP-7 exhibits phospholipase C activity, cleaving phosphocholine from palmitoyl lyso-phosphatidylcholine and platelet-activating factor (PAF), thereby inactivating PAF. Despite its enzymatic activities, ENPP-7 does not possess nucleotide pyrophosphatase activity. The protein's involvement in these processes suggests a potential role in promoting cholesterol absorption by modulating sphingomyelin levels derived from dietary or endogenous sources within the intestinal lumen.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q3TIW9 (A22-Q421)

Gene ID

238011  [NCBI]

Molecular Construction
N-term
ENPP-7 (A22-Q421)
Accession # Q3TIW9
His
C-term
Synonyms
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7; NPP-7; Alk-Smase
Molecular Weight

Approximately 60 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

ENPP-7 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ENPP-7 Protein, Mouse (HEK293, His)
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HY-P76902
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