EphA1 Protein, Human (HEK293, His)

The EphA1 protein is a receptor tyrosine kinase that promiscuously binds to ephrin-A ligands on neighboring cells, initiating contact-dependent bidirectional signaling. The forward signal originates from the receptor and the reverse signal originates from the ephrin ligand. EphA1 Protein, Human (HEK293, His) is the recombinant human-derived EphA1 protein, expressed by HEK293 , with C-6*His labeled tag.

The EphA1 protein, a receptor tyrosine kinase, engages in promiscuous binding to membrane-bound ephrin-A family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the pathway downstream of the ephrin ligand is termed reverse signaling. EphA1 exhibits low-affinity binding to EFNA3 and EFNA4 and high-affinity binding to EFNA1, which likely constitutes its cognate/functional ligand. Upon activation by EFNA1, EphA1 induces cell attachment to the extracellular matrix, inhibiting cell spreading and motility through the regulation of ILK and downstream RHOA and RAC. Additionally, EphA1 plays a role in angiogenesis and regulates cell proliferation. It may also be involved in apoptosis processes, contributing to its diverse cellular functions.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Ephrin-A1 (HY-P72662) is immobilized at 2 µg/mL(100 µL/well) can bind Human EphA1 (HY-P70321). The ED₅₀ for this effect is 17.1 ng/mL.

Human

HEK293

C-6*His

AAI30292.1 (K26-E547)

2041  [NCBI]

Partial

KEVTLMDTSKAQGELGWLLDPPKDGWSEQQQILNGTPLYMYQDCPMQGRRDTDHWLRSNWIYRGEEASRVHVELQFTVRDCKSFPGGAGPLGCKETFNLLYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGRLTRRGLYLAFHNPGACVALVSVRVFYQRCPETLNGLAQFPDTLPGPAGLVEVAGTCLPHARASPRPSGAPRMHCSPDGEWLVPVGRCHCEPGYEEGGSGEACVACPSGSYRMDMDTPHCLTCPQQSTAESEGATICTCESGHYRAPGEGPQVACTGPPSAPRNLSFSASGTQLSLRWEPPADTGGRQDVRYSVRCSQCQGTAQDGGPCQPCGVGVHFSPGARGLTTPAVHVNGLEPYANYTFNVEAQNGVSGLGSSGHASTSVSISMGHAESLSGLSLRLVKKEPRQLELTWAGSRPRSPGANLTYELHVLNQDEERYQMVLEPRVLLTELQPDTTYIVRVRMLTPLGPGPFSPDHEFRTSPPVSRGLTGGE

Approximately 70-85 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.