EphA2 Protein, Cynomolgus (HEK293, His-Avi)

EphA2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with ephrin A ligands, affecting migration, adhesion, proliferation and differentiation. EphA2 interacts with DSG1, inhibits ERK1/ERK2 signaling, and affects cell adhesion and UV radiation-induced apoptosis. EphA2 Protein, Cynomolgus (HEK293, His-Avi) is the recombinant cynomolgus-derived EphA2 protein, expressed by HEK293 , with C-Avi, C-His labeled tag.

EphA2 Protein is a receptor tyrosine kinase that interacts with membrane-bound ephrin-A ligands on adjacent cells, resulting in contact-dependent bidirectional signaling. This receptor mediates both forward signaling and reverse signaling pathways. Activation by ephrin-A1/EFNA1 regulates various cellular processes, including migration, integrin-mediated adhesion, proliferation, and differentiation. EphA2 also influences cell adhesion and differentiation through its interaction with DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. Additionally, it may play a role in UV radiation-induced apoptosis and stimulate chemotactic cell migration independently of ligand binding. During development, EphA2 is involved in pattern formation and contributes to the development of fetal tissues, such as angiogenesis, hindbrain development, and mammary gland morphogenesis. Its interaction with ephrin-A5/EFNA5 is crucial for maintaining lens transparency by regulating the shape and interactions of lens fiber cells. Furthermore, EphA2 participates in bone remodeling by regulating osteoclastogenesis and osteoblastogenesis through its interaction with ephrin-A2/EFNA2.

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant Cynomolgus EphA2 is immobilized at 2 µg/mL(100 µL/well) can bind Mouse Ephrin-A1. The ED₅₀ for this effect is 2.073 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized Recombinant Cynomolgus EphA2 is immobilized at 2 µg/mL(100 µL/well) can bind Mouse Ephrin-A1. The ED₅₀ for this effect is 2.073 ng/mL.

Cynomolgus

HEK293

C-Avi;C-8*His

Q1KL86 (A24-S534)

102146108  [NCBI]

Partial

AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQSLARFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEVSESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPMSMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCESSVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGS

60-70 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.