EphA2 Protein, Cynomolgus (HEK293, His-Avi)

EphA2 Protein, a receptor tyrosine kinase, engages in bidirectional signaling with ephrin-A ligands, influencing migration, adhesion, proliferation, and differentiation. EphA2 interacts with DSG1, inhibiting ERK1/ERK2 signaling, impacting cell adhesion and UV radiation-induced apoptosis. In development, EphA2 contributes to pattern formation, angiogenesis, hindbrain development, and mammary gland morphogenesis. Its interaction with Ephrin-A5 maintains lens transparency, and in bone remodeling, it regulates osteoclastogenesis and osteoblastogenesis through Ephrin-A2 interaction. EphA2 also acts as a receptor for hepatitis C virus in hepatocytes, facilitating viral entry and membrane fusion. EphA2 Protein, Cynomolgus (HEK293, His-Avi) is the recombinant cynomolgus-derived EphA2 protein, expressed by HEK293, with C-Avi, C-His labeled tag. The total length of EphA2 Protein, Cynomolgus (HEK293, His-Avi) is 511 a.a., with molecular weight of 60-70 kDa.

EphA2 Protein is a receptor tyrosine kinase that interacts with membrane-bound ephrin-A ligands on adjacent cells, resulting in contact-dependent bidirectional signaling. This receptor mediates both forward signaling and reverse signaling pathways. Activation by ephrin-A1/EFNA1 regulates various cellular processes, including migration, integrin-mediated adhesion, proliferation, and differentiation. EphA2 also influences cell adhesion and differentiation through its interaction with DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 signaling pathway. Additionally, it may play a role in UV radiation-induced apoptosis and stimulate chemotactic cell migration independently of ligand binding. During development, EphA2 is involved in pattern formation and contributes to the development of fetal tissues, such as angiogenesis, hindbrain development, and mammary gland morphogenesis. Its interaction with ephrin-A5/EFNA5 is crucial for maintaining lens transparency by regulating the shape and interactions of lens fiber cells. Furthermore, EphA2 participates in bone remodeling by regulating osteoclastogenesis and osteoblastogenesis through its interaction with ephrin-A2/EFNA2.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Cynomolgus

HEK293

C-Avi;C-His

Q1KL86 (A24-S534)

102146108  [NCBI]

60-70 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.