EPHA5 Protein, Human (HEK293, His)

The EPHA5 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It is involved in forward and reverse signaling pathways, and EFNA5 may serve as its cognate ligand. EPHA5 Protein, Human (HEK293, His) is the recombinant human-derived EPHA5 protein, expressed by HEK293 , with C-His labeled tag.

The EPHA5 protein, a receptor tyrosine kinase, engages in promiscuous binding to GPI-anchored ephrin-A family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is known as forward signaling, while the pathway downstream of the ephrin ligand is termed reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is likely the cognate/functional ligand for EPHA5. EPHA5 functions as an axon guidance molecule during development and may be involved in shaping the retinotectal, entorhino-hippocampal, and hippocamposeptal pathways. Together with EFNA5, it plays a role in synaptic plasticity in the adult brain by regulating synaptogenesis. Beyond its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells, contributing to the regulation of glucose-stimulated insulin secretion.

This product does not contain protein kinase domain.

Human

HEK293

C-8*His

P54756-1 (A26-P573)

2044  [NCBI]

Extracellular Domain

ASLAGCYSAPRRAPLWTCLLLCAALRTLLASPSNEVNLLDSRTVMGDLGWIAFPKNGWEEIGEVDENYAPIHTYQVCKVMEQNQNNWLLTSWISNEGASRIFIELKFTLRDCNSLPGGLGTCKETFNMYYFESDDQNGRNIKENQYIKIDTIAADESFTELDLGDRVMKLNTEVRDVGPLSKKGFYLAFQDVGACIALVSVRVYYKKCPSVVRHLAVFPDTITGADSSQLLEVSGSCVNHSVTDEPPKMHCSAEGEWLVPIGKCMCKAGYEEKNGTCQVCRPGFFKASPHIQSCGKCPPHSYTHEEASTSCVCEKDYFRRESDPPTMACTRPPSAPRNAISNVNETSVFLEWIPPADTGGRKDVSYYIACKKCNSHAGVCEECGGHVRYLPRQSGLKNTSVMMVDLLAHTNYTFEIEAVNGVSDLSPGARQYVSVNVTTNQAAPSPVTNVKKGKIAKNSISLSWQEPDRPNGIILEYEIKYFEKDQETSYTIIKSKETTITAEGLKPASVYVFQIRARTAAGYGVFSRRFEFETTPVFAASSDQSQIP

Approximately 68-80 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

≥ 95%, as determined by Bis-Tris PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.