EphA8 Protein, Human (HEK293, His)
Based on 1 Customer Validation
The EPHA8 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It participates in forward and reverse signaling pathways and is phosphorylated by ligands such as EFNA2, EFNA3, and EFNA5. EphA8 Protein, Human (HEK293, His) is the recombinant human-derived EphA8 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The EPHA8 protein is a receptor tyrosine kinase that promiscuously binds to the GPI-anchored ephrin-A ligand to initiate bidirectional signaling. It participates in forward and reverse signaling pathways and is phosphorylated by ligands such as EFNA2, EFNA3, and EFNA5. EphA8 Protein, Human (HEK293, His) is the recombinant human-derived EphA8 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The EphA8 protein, a receptor tyrosine kinase, engages in promiscuous binding to GPI-anchored ephrin-A family ligands on adjacent cells, initiating contact-dependent bidirectional signaling. The downstream pathway originating from the receptor is termed forward signaling, while the pathway downstream of the ephrin ligand is referred to as reverse signaling. GPI-anchored ephrin-A ligands, including EFNA2, EFNA3, and EFNA5, demonstrate the ability to activate EPHA8 through phosphorylation. EPHA8, in conjunction with EFNA5, may regulate integrin-mediated cell adhesion and migration on fibronectin substrates, as well as neurite outgrowth. In the development of the nervous system, EPHA8 also plays a crucial role in axon guidance. Downstream effectors of the EPHA8 signaling pathway include FYN, which promotes cell adhesion upon activation by EPHA8, and the MAP kinases, contributing to the stimulation of neurite outgrowth.
Verified Bioactivity
1.This product does not contain protein kinase domain.
2.Immobilized Recombinant Human EFNA5 (C-Fc) at 2 μg/mL (100 μL/well) can bind Recombinant Human EphA8 (C-6His).The ED50 of Recombinant Human EphA8 (C-6His) is 20-30 ng/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P29322-1 (E31-T542)
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Molecular Construction
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N-term
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EphA8 (E31-T542)
Accession # P29322-1 -
6*His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
EPHA8; EK3; Prev. EEK; Hydroxyaryl-Protein Kinase; Hek3; Protein-Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EEK; Tyrosylprotein Kinase; EPH- And ELK-Related Kinase; KIAA1459; Ephrin Type-A Receptor 8; EphA8; EPH-Like Kinase 3; HEK3; EPH Receptor A8
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AA Sequence
EVNLLDTSTIHGDWGWLTYPAHGWDSINEVDESFQPIHTYQVCNVMSPNQNNWLRTSWVPRDGARRVYAEIKFTLRDCNSMPGVLGTCKETFNLYYLESDRDLGASTQESQFLKIDTIAADESFTGADLGVRRLKLNTEVRSVGPLSKRGFYLAFQDIGACLAILSLRIYYKKCPAMVRNLAAFSEAVTGADSSSLVEVRGQCVRHSEERDTPKMYCSAEGEWLVPIGKCVCSAGYEERRDACVACELGFYKSAPGDQLCARCPPHSHSAAPAAQACHCDLSYYRAALDPPSSACTRPPSAPVNLISSVNGTSVTLEWAPPLDPGGRSDITYNAVCRRCPWALSRCEACGSGTRFVPQQTSLVQASLLVANLLAHMNYSFWIEAVNGVSDLSPEPRRAAVVNITTNQAAPSQVVVIRQERAGQTSVSLLWQEPEQPNGIILEYEIKYYEKDKEMQSYSTLKAVTTRATVSGLKPGTRYVFQVRARTSAGCGRFSQAMEVETGKPRPRYDTRT
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Predicted Molecular Mass
57.4 kDa
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Molecular Weight
Approximately 65-80 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Citrate, 6% trehalose, 50 mM NaCl, 0.05% Tween 80, pH 5.0.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (239 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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