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FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)

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Based on 1 Customer Validation

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)
  • FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

FCGRT is an IgG Fc receptor. Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. FCGRT plays a role in regulating IgG and serum albumin conversion. Overexpression of FCGRT is also associated with poor prognosis of tumors. FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived FCRN-B2M protein, expressed by HEK293 , with C-Avi, C-6*His labeled tag.

Background

IgG receptor FcRn large subunit p51 is an IgG Fc receptor with a molecular structure similar to MHC Class I and also binds to β-2 microglobulin. In rodents, FcRn was originally thought to be a receptor that trantranks maternal immunoglobulin G (IgG) from mother to newborn offspring via breast milk and is therefore known as a neonatal Fc receptor. FcRn has also been shown to play a role in regulating IgG and serum albumin conversion, and neonatal Fc receptor expression is up-regulated by pro-inflammatory cytokines TNF and down-regulated by IFN-γ. In the acidic endosomes of endothelial and hematopoietic cells, monomer IgG binds to FcRn, circulates IgG to the cell surface, where it is released into the circulation, regulating, in addition to IgG, homeostasis of the circulating protein albumin /ALB. The up-regulated expression of FCGRT in bladder cancer may serve as a key neutrophil gene associated with poor prognosis. FCGRT overexpression was also associated with decreased PD-L1 expression and decreased tumor mutation load (TMB) level[1][2][3][4][5][6].

Verified Bioactivity

1. Loaded Biotinylated Human FcRn Heterodimer-His-Avi on HIS1K Biosensor, can bind Anti-Human HER2 mAb (Human IgG1) with an affinity constant of 0.11 μM as determined in BLI assay.
2. Loaded Rozanolixizumab (HY-P9979) on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.430E-10 M as determined in BLI assay.

MCE Validation Data

  • Bioactivity - BLI

    Bioactivity - BLI

    Loaded Rozanolixizumab (HY-P9979) on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.430E-10 M as determined in BLI assay.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-6*His
  • Accession

    AAF72596 (A24-S297)&P61769 (I21-M119)

  • Gene ID
    2217  [NCBI]&567  [NCBI]
  • Molecular Construction
    • N-term
    • FCGRT (A24-S297)
      Accession # AAF72596
    • 6*His
    • C-term
    • N-term
    • B2M (I21-M119)
      Accession # P61769
    • Avi
    • C-term
  • Conjugation

    Biotin

  • Conjugate Method

    The single lysine residue in Avitag is biotinylated through an enzymatic reaction.

  • Synonyms

    FCGRT; Major Histocompatibility Complex Class I-Like Fc Receptor; Fc Gamma Receptor And Transporter; Neonatal Crystallizable Fc Receptor FcRn Splice Variant 6; Neonatal Fc Receptor; Neonatal Crystallizable Fc Receptor FcRn Splice Variant 5; FcRn; Neonatal Fragment Crystallizable Fc Receptor FcRn; IgG Fc Fragment Receptor Transporter Alpha Chain; Immunoglobulin Receptor, Intestinal, Heavy Chain; IgG Receptor FcRn Large Subunit P51; Neonatal Fc-Receptor For Ig; FcgammaRn; FCRN Alpha-Chain; Heavy Chain Of The Major Histocompatibility Complex Class I-Like Fc Receptor; FcRn Alpha Chain; Transmembrane Alpha Chain Of The Neonatal Receptor; Alpha-Chain; Fc Fragment Of IgG, Receptor, Transporter, Alpha; FCRN; Fc Fragment Of IgG Receptor And Transporter; B2M; Beta 2-Microglobulin; Beta-2-Microglobulin; Beta-2-Microglobin; Beta Chain Of MHC Class I Molecules; AMYLD6; Beta 2-Microglobulin Protein; MHC1D4; Human Nkt Tcr Alpha Chain; IMD43; Human Nkt Tcr Beta Chain

  • AA Sequence

    AESHLSLLYHLTAVSSPAPGTPAFWVSGWLGPQQYLSYNSLRGEAEPCGAWVWENQVSWYWEKETTDLRIKEKLFLEAFKALGGKGPYTLQGLLGCELGPDNTSVPTAKFALNGEEFMNFDLKQGTWGGDWPEALAISQRWQQQDKAANKELTFLLFSCPHRLREHLERGRGNLEWKEPPSMRLKARPSSPGFSVLTCSAFSFYPPELQLRFLRNGLAAGTGQGDFGPNSDGSFHASSSLTVKSGDEHHYCCIVQHAGLAQPLRVELESPAKSS&IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

  • Predicted Molecular Mass

    31.1 kDa & 13.5 kDa

  • Molecular Weight

    Approximately 35 kDa & 13 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 7.8.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. imister NE, et al. Cloning and expression of the neonatal rat intestinal Fc receptor, a major histocompatibility complex class I antigen homolog. Cold Spring Harb Symp Quant Biol. 1989;54 Pt 1:571-80. [Content Brief]

[2]. Ghetie V, et al. Abnormally short serum half-lives of IgG in beta 2-microglobulin-deficient mice. Eur J Immunol. 1996 Mar;26(3):690-6. [Content Brief]

[3]. Yang R, et al. Neutrophil-related genes predict prognosis and response to immune checkpoint inhibitors in bladder cancer. Front Pharmacol. 2022 Oct 21;13:1013672. [Content Brief]

[4]. West AP Jr, et al. Crystal structure and immunoglobulin G binding properties of the human major histocompatibility complex-related Fc receptor(,). Biochemistry. 2000 Aug 15;39(32):9698-708. [Content Brief]

[5]. Pyzik M, et al FcRn regulates albumin homeostasis and susceptibility to liver injury. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2862-E2871. [Content Brief]

[6]. Story CM, et al. A major histocompatibility complex class I-like Fc receptor cloned from human placenta: possible role in transfer of immunoglobulin G from mother to fetus. J Exp Med. 1994 Dec 1;180(6):2377-81. [Content Brief]

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Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Loaded Rozanolixizumab (HY-P9979) on AHC2 biosensor, can bind FCRN-B2M Protein, Human (Biotinylated, HEK293, His-Avi) with an affinity constant of 1.430E-10 M as determined in BLI assay.

  • FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) Product Image
    1/16
  • Experimental Validation Results for FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi)
  • FCRN-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His-Avi) Product Image