FSTL3 Protein, Mouse (HEK293, His)

In its secreted form, the FSTL3 protein acts as a binder and antagonist to TGF-β family members, inhibiting cell signaling induced by activin A, activin B, BMP2, and MSTN. It plays a role in bone formation, inhibition of osteoclast differentiation, hematopoiesis, and enhanced hematopoietic progenitor cell adhesion. FSTL3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.

The FSTL3 Protein, in its secreted form, acts as a binding and antagonizing agent for members of the TGF-beta family, such as activin, BMP2, and MSTN. It effectively inhibits cellular signaling induced by activin A, activin B, BMP2, and MSTN, with a higher efficacy observed for activin A. FSTL3 plays a role in bone formation by inhibiting osteoclast differentiation and contributes to hematopoiesis by influencing the differentiation of hemopoietic progenitor cells and enhancing their adhesion to fibronectin. Moreover, it appears to play a role in the adhesion of hematopoietic precursor cells to the bone marrow stroma. The nuclear form of FSTL3 likely participates in transcriptional regulation through its interaction with MLLT10. Additionally, FSTL3 interacts with INHBA, INHBB, FN1, ADAM12, and MSTN, further highlighting its diverse molecular interactions and multifaceted functional roles.

1.Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC₅₀ of >20.1 ng/mL determined by ELISA.
2.Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse INHBA, the ED₅₀ for this effect is 21.37 ng/mL.

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  Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse INHBA, The ED₅₀ for this effect is 21.37 ng/mL.

Mouse

HEK293

C-His

Q9EQC7 (V24-V256)

83554  [NCBI]

Full Length of Mature Protein

VMGSEDSVPGGVCWLQQGREATCSLVLKTRVSREECCASGNINTAWSNFTHPGNKISLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPHCECVPNCEGLPAGFQVCGSDGATYRDECELRTARCRGHPDLRVMYRGRCQKSCAQVVCPRPQSCLVDQTGSAHCVVCRAAPCPVPSNPGQELCGNNNVTYISSCHLRQATCFLGRSIGVRHPGICTGGPKVPAEEEENFV

35-40 kDa.

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.