FSTL3 Protein, Mouse (HEK293, His)
Based on 1 Customer Validation
In its secreted form, the FSTL3 protein acts as a binder and antagonist to TGF-β family members, inhibiting cell signaling induced by activin A, activin B, BMP2, and MSTN. It plays a role in bone formation, inhibition of osteoclast differentiation, hematopoiesis, and enhanced hematopoietic progenitor cell adhesion. FSTL3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Mouse
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
In its secreted form, the FSTL3 protein acts as a binder and antagonist to TGF-β family members, inhibiting cell signaling induced by activin A, activin B, BMP2, and MSTN. It plays a role in bone formation, inhibition of osteoclast differentiation, hematopoiesis, and enhanced hematopoietic progenitor cell adhesion. FSTL3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FSTL3 protein, expressed by HEK293 , with C-His labeled tag.
Background
The FSTL3 Protein, in its secreted form, acts as a binding and antagonizing agent for members of the TGF-beta family, such as activin, BMP2, and MSTN. It effectively inhibits cellular signaling induced by activin A, activin B, BMP2, and MSTN, with a higher efficacy observed for activin A. FSTL3 plays a role in bone formation by inhibiting osteoclast differentiation and contributes to hematopoiesis by influencing the differentiation of hemopoietic progenitor cells and enhancing their adhesion to fibronectin. Moreover, it appears to play a role in the adhesion of hematopoietic precursor cells to the bone marrow stroma. The nuclear form of FSTL3 likely participates in transcriptional regulation through its interaction with MLLT10. Additionally, FSTL3 interacts with INHBA, INHBB, FN1, ADAM12, and MSTN, further highlighting its diverse molecular interactions and multifaceted functional roles.
Verified Bioactivity
1.Immobilized Human Activin A, No Tag at 0.2 μg/mL (100 μl/well) on the plate. Dose response curve for Mouse FSTL3, His Tag with the EC50 of >20.1 ng/mL determined by ELISA.
2.Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/mL (100 μL/well) can bind biotinylated mouse INHBA, the ED50 for this effect is 21.37 ng/mL.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
-
Bioactivity - ELISA
Bioactivity - ELISA
Technical Parameters
-
Species Mouse
-
Source HEK293
-
Tag C-His
-
Accession
Q9EQC7 (V24-V256)
-
Molecular Construction
-
N-term
-
FSTL3 (V24-V256)
Accession # Q9EQC7 -
His
-
C-term
-
-
Protein Length
Full Length of Mature Protein
-
Synonyms
FSTL3; Follistatin-Related Gene Protein; Follistatin Like 3; Follistatin-Related Protein 3; FLRG; Follistatin-Like Protein 3; FSRP; Follistatin-Related Protein; Follistatin-Like 3 (Secreted Glycoprotein)
-
AA Sequence
VMGSEDSVPGGVCWLQQGREATCSLVLKTRVSREECCASGNINTAWSNFTHPGNKISLLGFLGLVHCLPCKDSCDGVECGPGKACRMLGGRPHCECVPNCEGLPAGFQVCGSDGATYRDECELRTARCRGHPDLRVMYRGRCQKSCAQVVCPRPQSCLVDQTGSAHCVVCRAAPCPVPSNPGQELCGNNNVTYISSCHLRQATCFLGRSIGVRHPGICTGGPKVPAEEEENFV
-
Molecular Weight
Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Glycosylation
Yes
-
Purity
≥ 95%, as determined by Bis-Tris PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (237 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
