FYN Protein, Human (Active, Biotinylated, sf9, His, Flag, Avi)
Based on 1 Customer Validation
FYN Protein, Human (Biotinylated, sf9, His, Flag, Avi) is the recombinant human-derived FYN, expressed by Sf9 insect cells , with Avi, His, Flag labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FYN Protein, Human (Biotinylated, sf9, His, Flag, Avi) is the recombinant human-derived FYN, expressed by Sf9 insect cells , with Avi, His, Flag labeled tag.
Background
Fyn is a non-receptor tyrosine-protein kinase involved in regulating diverse biological processes such as cell growth/survival, adhesion, integrin-mediated signaling, cytoskeletal remodeling, motility, immune response, and axon guidance. Inactive Fyn is phosphorylated at its C-terminal tail; upon PKA activation, it associates with PTK2/FAK1 to facilitate FAK1 phosphorylation and focal adhesion targeting. It modulates adhesion/motility via CTNNB1 (β-catenin) and CTNND1 (δ-catenin) phosphorylation, and drives cytoskeletal remodeling by phosphorylating WAS, MAP2, and MAPT. Fyn promotes cell survival by inhibiting AGAP2/PIKE-A cleavage and maintains glomerular slit diaphragm integrity via NPHS1/KIRREL1/TRPC6 phosphorylation. In neural contexts, it phosphorylates DPYSL2, ARHGAP32, and SNCA. Fyn also mediates TCR-induced T-cell proliferation/differentiation by phosphorylating PTK2B/PYK2 and negatively regulates TCR signaling through PAG1-CSK interactions. Similar roles include: DAB1 phosphorylation in reelin signaling (by similar); CLNK phosphorylation in IgE receptor-activated mast cells (by similar); and CD244-dependent NK cell activation.
Verified Bioactivity
The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. When the protein concentration is 0.0625 nM, the conversion rate is about 20%.
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-Avi;N-His;N-Flag
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Accession
P06241-1 (M1-L537)
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Gene ID2534
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Protein Length
Full Length of Isoform-1
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Conjugation
Biotin
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Synonyms
FYN; MGC45350; FYN Proto-Oncogene, Src Family Tyrosine Kinase; P59-Fyn; SLK; OKT3-Induced Calcium Influx Regulator; Tyrosine-Protein Kinase Fyn; Tyrosine Kinase P59fyn(T); Proto-Oncogene C-Fyn; Src/Yes-Related Novel; SYN; C-Syn Protooncogene; FYN Oncogene
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AA Sequence
MGCVQCKDKEATKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMNKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQSFLEDYFTATEPQYQPGENL
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Molecular Weight
Approximately 66 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 80%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 150 mM NaCl, 1 mM DTT, 10% glycerol.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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