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GGT1 Protein, Human (HEK293, His)

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Based on 1 publication(s) in Google Scholar

The GGT1 protein cleaves gamma-glutamyl bonds of extracellular glutathione, glutathione conjugates, and other gamma-glutamyl compounds. Its metabolism releases free glutamate and cysteinylglycine, which are hydrolyzed into cysteine and glycine. GGT1 Protein, Human (HEK293, His) is the recombinant human-derived GGT1 protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

The GGT1 protein cleaves gamma-glutamyl bonds of extracellular glutathione, glutathione conjugates, and other gamma-glutamyl compounds. Its metabolism releases free glutamate and cysteinylglycine, which are hydrolyzed into cysteine and glycine. GGT1 Protein, Human (HEK293, His) is the recombinant human-derived GGT1 protein, expressed by HEK293 , with N-His labeled tag.

Background

The GGT1 protein plays a pivotal role in cellular metabolism by cleaving the gamma-glutamyl bond in various substrates, including extracellular glutathione, glutathione conjugates such as maresin conjugate (13R)-S-glutathionyl-(14S)-hydroxy-(4Z,7Z,9E,11E,16Z,19Z)-docosahexaenoate (MCTR1), and other gamma-glutamyl compounds like leukotriene C4 (LTC4). This enzymatic activity results in the release of free glutamate and the dipeptide cysteinyl-glycine, which can be further hydrolyzed to cysteine and glycine by dipeptidases. Under conditions of elevated dipeptides and certain amino acids, GGT1 can catalyze a transpeptidation reaction, transferring the gamma-glutamyl moiety to an acceptor amino acid and forming a new gamma-glutamyl compound. Beyond its involvement in cysteine and glutathione homeostasis, GGT1 contributes to the conversion of leukotriene LTC4 to LTD4. It is noteworthy that GGT1 appears to be inactive under certain circumstances.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-8*His
  • Accession

    P19440-1 (P27-Y569)

  • Gene ID
    2678  [NCBI]
  • Molecular Construction
    • N-term
    • 8*His
    • GGT1 (P27-Y569)
      Accession # P19440-1
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    GGT1; GGT 1; Prev. GGT; Gamma-Glutamyltranspeptidase; D22S732; Gamma-Glutamyltransferase; D22S672; Glutathione Hydrolase; CD224; CD224 Antigen; Glutathione Hydrolase 1 Proenzyme; GGT1 Protein; Gamma-Glutamyltranspeptidase 1; GGTD; Leukotriene-C4 Hydrolase

  • AA Sequence

    PSASKEPDNHVYTRAAVAADAKQCSKIGRDALRDGGSAVDAAIAALLCVGLMNAHSMGIGGGLFLTIYNSTTRKAEVINAREVAPRLAFATMFNSSEQSQKGGLSVAVPGEIRGYELAHQRHGRLPWARLFQPSIQLARQGFPVGKGLAAALENKRTVIEQQPVLCEVFCRDRKVLREGERLTLPQLADTYETLAIEGAQAFYNGSLTAQIVKDIQAAGGIVTAEDLNNYRAELIEHPLNISLGDVVLYMPSAPLSGPVLALILNILKGYNFSRESVESPEQKGLTYHRIVEAFRFAYAKRTLLGDPKFVDVTEVVRNMTSEFFAAQLRAQISDDTTHPISYYKPEFYTPDDGGTAHLSVVAEDGSAVSATSTINLYFGSKVRSPVSGILFNNEMDDFSSPSITNEFGVPPSPANFIQPGKQPLSSMCPTIMVGQDGQVRMVVGAAGGTQITTATALAIIYNLWFGYDVKRAVEEPRLHNQLLPNVTTVERNIDQAVTAALETRHHHTQIASTFIAVVQAIVRTAGGWAAASDSRKGGEPAGY

  • Predicted Molecular Mass

    59.7 kDa

  • Molecular Weight

    Approximately 50-65 kDa (heavy chain) & 23-30 kDa (light chain), based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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