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GOT1 Protein, Human (His)

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Based on 1 publication(s) in Google Scholar

GOT1 Protein, Human (His), a recombinant human GOT1 produced in E. coli, has a His tag. Calcitonin has an effect on decreasing osteoclast activity and has the potential for hypercalcemia research. Reduction in glutamate concentration in blood is induced through the activity of the blood-resident enzyme glutamate oxaloacetate transaminase 1 (GOT1), which catalyzes the reversible transformation of oxaloacetate and glutamate to aspartate and α-ketoglutarate.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for GOT1 Protein, Human (His)
  • GOT1 Protein, Human (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

GOT1 Protein, Human (His), a recombinant human GOT1 produced in E. coli, has a His tag. Calcitonin has an effect on decreasing osteoclast activity and has the potential for hypercalcemia research. Reduction in glutamate concentration in blood is induced through the activity of the blood-resident enzyme glutamate oxaloacetate transaminase 1 (GOT1), which catalyzes the reversible transformation of oxaloacetate and glutamate to aspartate and α-ketoglutarate[1].

Background

Blood glutamate scavenging is a novel and attractive protecting strategy to reduce the excitotoxic effect of extracellular glutamate released during ischemic brain injury. Glutamate oxaloacetate transaminase 1 (GOT1) activation by means of oxaloacetate administration has been used to reduce the glutamate concentration in the blood[1].

Verified Bioactivity

Specific activity is 194.189 units/mg, and is defined as the amount of enzyme that converts 1 umole of a-ketoglutarate to L-Glutamate per minute at pH 8.0 at 25°C.

Technical Parameters

  • Species Human
  • Source E. coli
  • Tag C-6*His
  • Accession

    P17174-1 (A2-Q413)

  • Gene ID
    2805  [NCBI]
  • Molecular Construction
    • N-term
    • GOT1 (A2-Q413)
      Accession # P17174
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1 Mature Protein

  • Synonyms

    GOT1; Aspartate Transaminase 1; Glutamic-Oxaloacetic Transaminase 1; Transaminase A; Glutamate Oxaloacetate Transaminase 1; CAspAT; AST1; CCAT; SGOT; Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1); AST; Testis Secretory Sperm-

  • AA Sequence

    APPSVFAEVPQAQPVLVFKLTADFREDPDPRKVNLGVGAYRTDDCHPWVLPVVKKVEQKIANDNSLNHEYLPILGLAEFRSCASRLALGDDSPALKEKRVGGVQSLGGTGALRIGADFLARWYNGTNNKNTPVYVSSPTWENHNAVFSAAGFKDIRSYRYWDAEKRGLDLQGFLNDLENAPEFSIVVLHACAHNPTGIDPTPEQWKQIASVMKHRFLFPFFDSAYQGFASGNLERDAWAIRYFVSEGFEFFCAQSFSKNFGLYNERVGNLTVVGKEPESILQVLSQMEKIVRITWSNPPAQGARIVASTLSNPELFEEWTGNVKTMADRILTMRSELRARLEALKTPGTWNHITDQIGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 8.0, 20% glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

References

[1]. M Pérez-Mato, et al. Human recombinant glutamate oxaloacetate transaminase 1 (GOT1) supplemented with oxaloacetate induces a protective effect after cerebral ischemia. Cell Death Dis. 2014 Jan 9;5(1):e992. [Content Brief]

Calculators

Reconstitution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • GOT1 Protein, Human (His) Product Image
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  • Experimental Validation Results for GOT1 Protein, Human (His)
  • GOT1 Protein, Human (His) Product Image