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GPD1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

GPD1 protein, a glycerol-3-phosphate dehydrogenase, crucially converts glycerol-3-phosphate to dihydroxyacetone phosphate, a key step in glycolysis and gluconeogenesis. Its enzymatic activity regulates cellular energy metabolism and glycerolipid biosynthesis, essential for maintaining cellular homeostasis and energy production. GPD1's role makes it a key player in metabolic processes with implications for various physiological functions. GPD1 Protein, Human (HEK293, His) is the recombinant human-derived GPD1 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for GPD1 Protein, Human (HEK293, His)
  • GPD1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

GPD1 protein, a glycerol-3-phosphate dehydrogenase, crucially converts glycerol-3-phosphate to dihydroxyacetone phosphate, a key step in glycolysis and gluconeogenesis. Its enzymatic activity regulates cellular energy metabolism and glycerolipid biosynthesis, essential for maintaining cellular homeostasis and energy production. GPD1's role makes it a key player in metabolic processes with implications for various physiological functions. GPD1 Protein, Human (HEK293, His) is the recombinant human-derived GPD1 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

GPD1 protein serves as a glycerol-3-phosphate dehydrogenase, playing a crucial role in the metabolic conversion of glycerol-3-phosphate. This enzymatic activity involves the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate, a key step in glycolysis and gluconeogenesis pathways. By catalyzing this reaction, GPD1 contributes to the regulation of cellular energy metabolism and the balance of glycerolipid biosynthesis. The glycerol-3-phosphate dehydrogenase activity of GPD1 is essential for maintaining cellular homeostasis and energy production, making it a key player in metabolic processes with implications for various physiological functions.

Verified Bioactivity

Measured by its ability to catalyze dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate. The specific activity is > 500 pmol/min/ug.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P21695-1 (M1-M349)

  • Gene ID
    2819  [NCBI]
  • Molecular Construction
    • N-term
    • GPD1 (M1-M349)
      Accession # P21695-1
    • 6*His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    GPD1; Glycerol-3-Phosphate Dehydrogenase [NAD+], Cytoplasmic; Glycerol-3-Phosphate Dehydrogenase 1; Glycerol-3-Phosphate Dehydrogenase [NAD(+)]; Glycerol-3-Phosphate Dehydrogenase [NAD(+)], Cytoplasmic; Epididymis Secretory Sperm Binding Protein; Glycerol

  • AA Sequence

    MASKKVCIVGSGNWGSAIAKIVGGNAAQLAQFDPRVTMWVFEEDIGGKKLTEIINTQHENVKYLPGHKLPPNVVAVPDVVQAAEDADILIFVVPHQFIGKICDQLKGHLKANATGISLIKGVDEGPNGLKLISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGPVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM

  • Predicted Molecular Mass

    38.6 kDa

  • Molecular Weight

    Approximately 32-48 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • GPD1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for GPD1 Protein, Human (HEK293, His)
  • GPD1 Protein, Human (HEK293, His) Product Image