GSDME Protein, Human (His)
Based on 1 Customer Validation
GSDME Protein, Human (His) is the recombinant human-derived GSDME protein, expressed by E. coli, with N-6*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
GSDME Protein, Human (His) is the recombinant human-derived GSDME protein, expressed by E. coli, with N-6*His tag.
Background
DFNA5 is a precursor of a pore-forming protein that converts non-inflammatory apoptosis to pyroptosis. Its N-terminal moiety (Gasdermin-E, N-terminal) binds to membranes and forms pores upon cleavage, triggering pyroptosis. The protein can be cleaved by CASP3 or granzyme B (GZMB), promoting the switch from apoptosis to pyroptosis or granzyme-mediated pyroptosis, respectively. After cleavage, DFNA5 translocates to the plasma membrane, forms pores (10-15 nm in diameter), and releases mature interleukins (e.g., IL1B and IL16) to induce pyroptosis. It also binds to bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate. CASP3-mediated cleavage enables DFNA5 to convert TNF- or chemotherapy-induced apoptosis into pyroptosis. Additionally, DFNA5 mediates secondary necrosis downstream of mitochondrial apoptosis and viral infections, exhibits bactericidal activity, and suppresses tumors via GZMB-triggered pyroptosis in NK cell targets. Similar roles include: involvement in p53/TP53-regulated DNA damage response. Microbial infection-related function: DFNA5 promotes maternal placental pyroptosis during Zika virus infection, contributing to adverse fetal outcomes.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-6*His
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Accession
O60443-1 (M1-S496)
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Gene ID1687
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Molecular Construction
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N-term
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6*His
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GSDME (M1-S496)
Accession # O60443-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
GSDME; Non-Syndromic Hearing Impairment Protein 5; Prev. DFNA5; Inversely Correlated With Estrogen Receptor Expression; ICERE-1; Nonsyndromic Hearing Impairment Protein; Inversely Correlated With Estrogen Receptor Expression 1; Deafness, Autosomal Dominan
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AA Sequence
MFAKATRNFLREVDADGDLIAVSNLNDSDKLQLLSLVTKKKRFWCWQRPKYQFLSLTLGDVLIEDQFPSPVVVESDFVKYEGKFANHVSGTLETALGKVKLNLGGSSRVESQSSFGTLRKQEVDLQQLIRDSAERTINLRNPVLQQVLEGRNEVLCVLTQKITTMQKCVISEHMQVEEKCGGIVGIQTKTVQVSATEDGNVTKDSNVVLEIPAATTIAYGVIELYVKLDGQFEFCLLRGKQGGFENKKRIDSVYLDPLVFREFAFIDMPDAAHGISSQDGPLSVLKQATLLLERNFHPFAELPEPQQTALSDIFQAVLFDDELLMVLEPVCDDLVSGLSPTVAVLGELKPRQQQDLVAFLQLVGCSLQGGCPGPEDAGSKQLFMTAYFLVSALAEMPDSAAALLGTCCKLQIIPTLCHLLRALSDDGVSDLEDPTLTPLKDTERFGIVQRLFASADISLERLKSSVKAVILKDSKVFPLLLCITLNGLCALGREHS
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Predicted Molecular Mass
57.3 kDa
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Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
2.Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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