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HMGN3 Protein, Human (His)

Cat. No.: HY-P75810
Handling Instructions

HMGN3, binding to nucleosomes, regulates chromatin structure, impacting chromatin-dependent processes like transcription and DNA repair. It influences insulin and glucagon levels, modulating pancreatic gene expression related to insulin secretion. HMGN3 specifically binds to the promoter region of the glucose transporter SLC2A2, recruiting PDX1 and other transcription factors. It also regulates the expression of SLC6A9, a glycine transporter in the central nervous system. Additionally, HMGN3 may play a role in ocular development and astrocyte function. HMGN3 Protein, Human (His) is the recombinant human-derived HMGN3 protein, expressed by E. coli , with N-His labeled tag. The total length of HMGN3 Protein, Human (His) is 77 a.a., with molecular weight of ~16 kDa.

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Description

HMGN3, binding to nucleosomes, regulates chromatin structure, impacting chromatin-dependent processes like transcription and DNA repair. It influences insulin and glucagon levels, modulating pancreatic gene expression related to insulin secretion. HMGN3 specifically binds to the promoter region of the glucose transporter SLC2A2, recruiting PDX1 and other transcription factors. It also regulates the expression of SLC6A9, a glycine transporter in the central nervous system. Additionally, HMGN3 may play a role in ocular development and astrocyte function. HMGN3 Protein, Human (His) is the recombinant human-derived HMGN3 protein, expressed by E. coli , with N-His labeled tag. The total length of HMGN3 Protein, Human (His) is 77 a.a., with molecular weight of ~16 kDa.

Background

HMGN3, a protein with the capability to bind to nucleosomes, exerts regulatory control over chromatin structure, thereby influencing various chromatin-dependent processes, including transcription, DNA replication, and DNA repair. It plays a role in the modulation of insulin and glucagon levels and participates in the regulation of pancreatic genes involved in insulin secretion. HMGN3 specifically binds to the promoter region of the glucose transporter SLC2A2, recruiting transcription factors such as PDX1, thereby impacting glucose homeostasis. Additionally, it regulates the expression of SLC6A9, a glycine transporter crucial for maintaining glycine concentration in synaptic junctions within the central nervous system. Beyond its metabolic functions, HMGN3 may also contribute to ocular development and astrocyte function. Furthermore, HMGN3 engages in interactions with the ligand binding domain of the thyroid receptor, requiring the presence of thyroid hormone for its activity, and interacts with the transcriptional regulator SEHBP, highlighting its multifaceted roles in cellular and physiological processes.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q15651-2 (M1-N77)

Gene ID
Molecular Construction
N-term
His
HMGN3 (M1-N77)
Accession # Q15651-2
C-term
Synonyms
High mobility group nucleosome-binding domain-containing protein 3; TRIP-7; HMGN3
Molecular Weight

Approximately 16 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HMGN3 Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HMGN3 Protein, Human (His)
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HY-P75810
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