HO-2/HMOX2 Protein, Human (His)

Through its catalytic activity, HO-2/HMOX2 protein promotes the oxidative cleavage of heme at the α-methylene bridge carbon, thereby releasing carbon monoxide (CO). This process simultaneously produces biliverdin IXalpha and releases the central heme iron chelate in the form of ferrous iron. HO-2/HMOX2 Protein, Human (His) is the recombinant human-derived HO-2/HMOX2 protein, expressed by E. coli , with C-6*His labeled tag. The total length of HO-2/HMOX2 Protein, Human (His) is 290 a.a., with molecular weight of ~31 & 35 kDa, respectively.

Heme oxygenase-2 (HO-2), encoded by the HMOX2 gene, is a critical enzyme that catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, resulting in the release of carbon monoxide (CO) and the generation of biliverdin IXalpha. Simultaneously, the enzyme liberates the central heme iron chelate as ferrous iron. This reaction represents a pivotal step in heme catabolism and serves as a regulatory mechanism for maintaining cellular heme homeostasis. The release of carbon monoxide and biliverdin, along with the liberation of ferrous iron, underscores the multifunctional role of HO-2 in both gasotransmitter signaling and the recycling of iron. The enzymatic activity of HO-2 is essential for various physiological processes, including antioxidant defense, anti-inflammatory responses, and the regulation of vascular tone.

Measured by its ability to oxidize hemin to biliverdin. The specific activity is >3.5 pmol/min/μg.

Human

E. coli

C-6*His

P30519 (S2-L291)

3163  [NCBI]

SAEVETSEGVDESEKKNSGALEKENQMRMADLSELLKEGTKEAHDRAENTQFVKDFLKGNIKKELFKLATTALYFTYSALEEEMERNKDHPAFAPLYFPMELHRKEALTKDMEYFFGENWEEQVQCPKAAQKYVERIHYIGQNEPELLVAHAYTRYMGDLSGGQVLKKVAQRALKLPSTGEGTQFYLFENVDNAQQFKQLYRARMNALDLNMKTKERIVEEANKAFEYNMQIFNELDQAGSTLARETLEDGFPVHDGKGDMRKCPFYAAEQDKGALEGSSCPFRTAMAVL

Approximately 31 & 35 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.