HSP60 Protein, Mouse (His, solution)
Based on 1 publication(s) in Google Scholar
The HSP60 protein, together with Hsp10, contributes to the import and assembly of mitochondrial proteins. It ensures correct folding and prevents incorrect folding under stressful conditions. HSP60 Protein, Mouse (His, solution) is the recombinant mouse-derived HSP60 protein, expressed by E. coli , with N-His labeled tag.
- Species: Mouse
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
The HSP60 protein, together with Hsp10, contributes to the import and assembly of mitochondrial proteins. It ensures correct folding and prevents incorrect folding under stressful conditions. HSP60 Protein, Mouse (His, solution) is the recombinant mouse-derived HSP60 protein, expressed by E. coli , with N-His labeled tag.
Background
The HSP60 protein plays a crucial role in mitochondrial protein import and macromolecular assembly. Along with Hsp10, it facilitates the proper folding of imported proteins and may also prevent misfolding while promoting the refolding and appropriate assembly of unfolded polypeptides generated during stressful conditions in the mitochondrial matrix. These chaperonins consist of heptameric rings formed by the large subunit Hsp60, which align in a back-to-back double ring configuration. Through a cyclic process, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by ATP binding and association with two heptameric rings of the co-chaperonin Hsp10. This leads to the encapsulation of the substrate protein within the inner cavity of Hsp60, allowing it to fold undisturbed by other cellular components for a certain period. Synchronized ATP hydrolysis in all Hsp60 subunits causes the dissociation of the chaperonin rings, releasing ADP and the properly folded substrate protein.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Mouse
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Source E. coli
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Tag N-His
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Accession
P63038 (L2-F573)
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Protein Length
Full Length
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Synonyms
HSPD1; HuCHA60; Prev. SPG13; CPN60; HSP60; Spastic Paraplegia 13 (Autosomal Dominant); 60 KDa Heat Shock Protein, Mitochondrial; Epididymis Secretory Sperm Binding Protein; Mitochondrial Matrix Protein P1; Heat Shock 60kD Protein 1 (Chaperonin); 60 KDa Chaperonin; Heat Shock 60kDa Protein 1 Isoform 2; Chaperonin 60; Heat Shock 60kDa Protein 1 Isoform 4; GroEL; Short Heat Shock Protein 60 Hsp60s1; Heat Shock 60kDa Protein 1 (Chaperonin); Heat Shock Protein 65; Heat Shock Protein Family D Member 1; HSP65; P60 Lymphocyte Protein; Hsp60; Heat Shock Protein 60; HLD4; Heat Shock Protein Family D (Hsp60) Member 1; HSP-60
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AA Sequence
LRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF
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Predicted Molecular Mass
62.3 kDa
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Molecular Weight
Approximately 58-65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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