HSP60 Protein, Mouse (His)

The HSP60 protein, together with Hsp10, contributes to the import and assembly of mitochondrial proteins. It ensures correct folding and prevents incorrect folding under stressful conditions. HSP60 Protein, Mouse (His) is the recombinant mouse-derived HSP60 protein, expressed by E. coli , with N-His labeled tag. The total length of HSP60 Protein, Mouse (His) is 572 a.a., with molecular weight of ~58 kDa.

The HSP60 protein plays a crucial role in mitochondrial protein import and macromolecular assembly. Along with Hsp10, it facilitates the proper folding of imported proteins and may also prevent misfolding while promoting the refolding and appropriate assembly of unfolded polypeptides generated during stressful conditions in the mitochondrial matrix. These chaperonins consist of heptameric rings formed by the large subunit Hsp60, which align in a back-to-back double ring configuration. Through a cyclic process, Hsp60 ring complexes bind one unfolded substrate protein per ring, followed by ATP binding and association with two heptameric rings of the co-chaperonin Hsp10. This leads to the encapsulation of the substrate protein within the inner cavity of Hsp60, allowing it to fold undisturbed by other cellular components for a certain period. Synchronized ATP hydrolysis in all Hsp60 subunits causes the dissociation of the chaperonin rings, releasing ADP and the properly folded substrate protein.

Mouse

E. coli

N-His

P63038 (L2-F573)

15510  [NCBI]

LRLPTVLRQMRPVSRALAPHLTRAYAKDVKFGADARALMLQGVDLLADAVAVTMGPKGRTVIIEQSWGSPKVTKDGVTVAKSIDLKDKYKNIGAKLVQDVANNTNEEAGDGTTTATVLARSIAKEGFEKISKGANPVEIRRGVMLAVDAVIAELKKQSKPVTTPEEIAQVATISANGDKDIGNIISDAMKKVGRKGVITVKDGKTLNDELEIIEGMKFDRGYISPYFINTSKGQKCEFQDAYVLLSEKKISSVQSIVPALEIANAHRKPLVIIAEDVDGEALSTLVLNRLKVGLQVVAVKAPGFGDNRKNQLKDMAIATGGAVFGEEGLNLNLEDVQAHDLGKVGEVIVTKDDAMLLKGKGDKAHIEKRIQEITEQLDITTSEYEKEKLNERLAKLSDGVAVLKVGGTSDVEVNEKKDRVTDALNATRAAVEEGIVLGGGCALLRCIPALDSLKPANEDQKIGIEIIKRALKIPAMTIAKNAGVEGSLIVEKILQSSSEVGYDAMLGDFVNMVEKGIIDPTKVVRTALLDAAGVASLLTTAEAVVTEIPKEEKDPGMGAMGGMGGGMGGGMF

Approximately 58 kDa

Greater than 85% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.