IGFBP-4 Protein, Human (HEK293, His)

IGFBP-4 Protein, Human (HEK293, His) is a member of the insulin-like growth factor-binding protein (IGFBP) family, binds to insulin-like growth factors, mediates IGF-independent biologic activities.

The insulin-like growth factor-binding protein (IGFBP) family consists of six different protein members (IGFBP1-6) that show high inhibition for IGF-1 and IGF-2. IGFBP-4 is a smallest member and modulates the biologic functions by binding to IGFs. Moreover, IGFBP-4 mediates IGF-independent biologic activities. IGFBP-4 inhibits colorectal cancer, breast cancer and glioblastoma cells growth. IGFBP-4 acts a crucial inflammatory mediator in the occurrence of severities acute respiratory distress syndrome, which is responsible for cancer proliferation, migration and apoptosis^[1].

1.The ED₅₀ is <50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15.0 ng/ml human IGF-II.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells.The ED₅₀ is 0.03-0.18 μg/mL in the presence of 14 ng/mL rhIGF-II.

• 
  Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells. The ED₅₀ for this effect is 0.1768 μg/mL in the presence of 14 ng/mL rhIGF-II, corresponding to a specific activity is 5656.1 units/mg.

Human

HEK293

C-6*His

P22692-1 (D22-E258)

3487  [NCBI]

Full Length of Isoform-1 Mature Protein

DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE

Approximately 30-35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Li W, et al. Insulin-like growth factor binding protein-4 inhibits cell growth, migration and invasion, and downregulates COX-2 expression in A549 lung cancer cells. Cell Biol Int. 2017 Apr;41(4):384-391.  [Content Brief]