IGFBP-4 Protein, Human (HEK293, His)
Based on 1 Customer Validation
IGFBP-4 Protein, Human (HEK293, His) is a member of the insulin-like growth factor-binding protein (IGFBP) family, binds to insulin-like growth factors, mediates IGF-independent biologic activities.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
IGFBP-4 Protein, Human (HEK293, His) is a member of the insulin-like growth factor-binding protein (IGFBP) family, binds to insulin-like growth factors, mediates IGF-independent biologic activities.
The insulin-like growth factor-binding protein (IGFBP) family consists of six different protein members (IGFBP1-6) that show high inhibition for IGF-1 and IGF-2. IGFBP-4 is a smallest member and modulates the biologic functions by binding to IGFs. Moreover, IGFBP-4 mediates IGF-independent biologic activities. IGFBP-4 inhibits colorectal cancer, breast cancer and glioblastoma cells growth. IGFBP-4 acts a crucial inflammatory mediator in the occurrence of severities acute respiratory distress syndrome, which is responsible for cancer proliferation, migration and apoptosis[1].
1.The ED50 is <50 ng/mL, measured in a bioassay using FDC-P1 cells in the presence of 15.0 ng/ml human IGF-II.
2. Measured by its ability to inhibit the biological activity of IGF-II on MCF-7 human breast cancer cells.The ED50 is 0.03-0.18 μg/mL in the presence of 14 ng/mL rhIGF-II.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P22692-1 (D22-E258)
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Molecular Construction
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N-term
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IGFBP-4 (D22-E258)
Accession # P22692-1 -
6*His
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C-term
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Protein Length
Full Length of Isoform-1 Mature Protein
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Synonyms
IGFBP4; IGF-Binding Protein 4; Insulin Like Growth Factor Binding Protein 4; HT29-IGFBP; IGFBP-4; BP-4; IBP4; IBP-4; Insulin-Like Growth Factor-Binding Protein 4; Insulin-Like Growth Factor Binding Protein 4
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AA Sequence
DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE
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Predicted Molecular Mass
26.8 kDa
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Molecular Weight
Approximately 30-35 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (262 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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