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IGFBP-5 Protein, Human (HEK293, His)

2 Cited Publications
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Based on 2 publication(s) in Google Scholar

IGFBP-5 Protein, Human (HEK293, His), Human is a unique insulin-like growth factor-binding protein, modulates the actions of secreted insulin-like growth factors, and stimulates osteoblast proliferation.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
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Biological Activity

Description

IGFBP-5 Protein, Human (HEK293, His), Human is a unique insulin-like growth factor-binding protein, modulates the actions of secreted insulin-like growth factors, and stimulates osteoblast proliferation.

Background

Insulin-like Growth Factor-Binding Proteins (IGFBPs) modulate the actions of secreted insulin-like growth facts (IGFs) by binding to them and increase the IGF halflife in the extracellular milieu and circulation by sequestering them in this bound form. IGFBPs either enhance or inhibit IGF actions on target cells; the individual IGFBPs either inhibit or potentiate IGF effects on osteoblasts in bone. Human Insulin-like Growth Factor-Binding Protein 5 (rhIGFBP-5) is a unique and most abundant IGFBP stored in bone, having a high specific binding affinity for hydroxyapatite and extracellular matrix proteins, therefore fixing it and its bound IGFs within bone. rhIGFBP-5 consistently stimulates osteoblast cell proliferation in vitro, thus increasing the number of osteoblasts[1].

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P24593 (L21-E272)

  • Gene ID
    3488  [NCBI]
  • Molecular Construction
    • N-term
    • IGFBP-5 (L21-E272)
      Accession # P24593
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IGFBP5; IBP-5; Insulin Like Growth Factor Binding Protein 5; IBP5; Insulin-Like Growth Factor-Binding Protein 5; Insulin-Like Growth Factor Binding Protein 5; IGF-Binding Protein 5; IGFBP5 Protein; IGFBP-5

  • AA Sequence

    LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

  • Predicted Molecular Mass

    29.6 kDa

  • Molecular Weight

    Approximately 30-40 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Richman C, et al. Recombinant human insulin-like growth factor-binding protein-5 stimulates bone formation parameters in vitro and in vivo. Endocrinology. 1999 Oct;140(10):4699-705. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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