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IGFBP-5 Protein, Human (sf9, His)

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The IGFBP-5 protein regulates insulin-like growth factor (IGF) and extends its lifespan. It can inhibit or stimulate the growth-promoting effect of IGF, showing its dual regulatory function. IGFBP-5 Protein, Human (sf9, His) is the recombinant human-derived IGFBP-5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: Sf9 insect cells
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The IGFBP-5 protein regulates insulin-like growth factor (IGF) and extends its lifespan. It can inhibit or stimulate the growth-promoting effect of IGF, showing its dual regulatory function. IGFBP-5 Protein, Human (sf9, His) is the recombinant human-derived IGFBP-5 protein, expressed by Sf9 insect cells , with C-His labeled tag.

Background

IGFBP-5 protein assumes a crucial role in regulating the activity of insulin-like growth factors (IGFs) by extending their half-life. Within cell culture, IGFBP-5 has demonstrated the ability to either inhibit or stimulate the growth-promoting effects of IGFs. This dual regulatory function underscores the complexity of IGFBP-5's influence on cellular processes. Importantly, IGFBP-5 achieves these effects by modulating the interaction between IGFs and their cell surface receptors, thereby fine-tuning the signaling pathways associated with growth and development. The intricate interplay between IGFBP-5 and IGFs highlights the nuanced control mechanisms that govern cellular responses to growth factors, offering insights into the multifaceted role of IGFBP-5 in the regulation of cellular growth and proliferation.

Verified Bioactivity

Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 μg/mL in the presence of 14 ng/mL rhIGF-II.

Technical Parameters

  • Species Human
  • Source Sf9 insect cells
  • Tag C-His
  • Accession

    P24593/NP_000590.1 (L21-E272)

  • Gene ID
    3488  [NCBI]
  • Molecular Construction
    • N-term
    • IGFBP-5 (L21-E272)
      Accession # P24593/NP_000590.1
    • His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    IGFBP5; IBP-5; Insulin Like Growth Factor Binding Protein 5; IBP5; Insulin-Like Growth Factor-Binding Protein 5; Insulin-Like Growth Factor Binding Protein 5; IGF-Binding Protein 5; IGFBP5 Protein; IGFBP-5

  • AA Sequence

    LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVE

  • Predicted Molecular Mass

    30 kDa

  • Molecular Weight

    Approximately 35-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, pH 8.0, 300 mM NaCl, 10% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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