IGFBP-5 Protein, Mouse (HEK293, His)

IGFBP-5 protein is a member of the IGFBP family that significantly prolongs the half-life of IGF and modulates its growth-promoting effects in cell culture. The multifunctional IGFBP-5 can inhibit or stimulate cellular responses to IGF by altering the kinetics of interaction with IGF receptors. IGFBP-5 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGFBP-5 protein, expressed by HEK293 , with C-6*His labeled tag.

IGFBP-5 Protein, a member of the insulin-like growth factor-binding proteins (IGFBPs), assumes a crucial role in extending the half-life of insulin-like growth factors (IGFs) and modulating their growth-promoting effects on cell culture. Demonstrating versatility, IGFBP-5 has been shown to either inhibit or stimulate the cellular responses elicited by IGFs. This regulatory function is achieved by IGFBP-5 through its ability to alter the interaction dynamics between IGFs and their corresponding cell surface receptors. By exerting control over these molecular interactions, IGFBP-5 contributes to the intricate modulation of IGF signaling pathways, influencing cellular responses related to growth and development. The diverse effects of IGFBP-5 underscore its significance in the regulatory framework that governs the interplay between IGFs and their receptors.

Mouse

HEK293

C-6*His

Q07079 (L20-E271)

16011  [NCBI]

Full Length of Mature Protein

LGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYGEQTKIERDSREHEEPTTSEMAEETYSPKVFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRVIPAPEMRQESEQGPCRRHMEASLQEFKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHAFDSSNVE

Approximately 38.0 kDa

≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.