IGSF3 Protein, Human (HEK293, His)
Based on 1 Customer Validation
IGSF3 Protein, an immunoglobulin-like superfamily member, has a Glu-Trp-Ile (EWI) motif in extracellular region. IGSF3 is a developmentally regulated protein that controls neuronal morphogenesis. IGSF3 controls the differentiation of granule cells, axonal growth and branching. IGSF3 is a novel regulator of neuronal morphogenesis that might function through interactions with multiple partners including the tetraspanin TSPAN7. IGSF3 functions as a tumor promoter and induces hepatocellular carcinoma (HCC) cancer cell growth and invasion by acting through the NF-κB signaling pathways. IGSF3 Protein, Human (HEK293, His) is the recombinant human-derived IGSF3 protein, expressed by HEK293 , with C-His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
IGSF3 Protein, an immunoglobulin-like superfamily member, has a Glu-Trp-Ile (EWI) motif in extracellular region. IGSF3 is a developmentally regulated protein that controls neuronal morphogenesis. IGSF3 controls the differentiation of granule cells, axonal growth and branching. IGSF3 is a novel regulator of neuronal morphogenesis that might function through interactions with multiple partners including the tetraspanin TSPAN7. IGSF3 functions as a tumor promoter and induces hepatocellular carcinoma (HCC) cancer cell growth and invasion by acting through the NF-κB signaling pathways[1][2]. IGSF3 Protein, Human (HEK293, His) is the recombinant human-derived IGSF3 protein, expressed by HEK293 , with C-His labeled tag.
IGSF3 is a membrane protein expressed in a neuron specific- and time-dependent manner during brain development. In the developing brain, IGSF3 is in a complex with the tetraspanin TSPAN7, a membrane protein mutated in several forms of X-linked intellectual disabilities[1].
IGSF3, situated on chromosome 1p13.1, is part of the immunoglobulin (Ig) superfamily. IGSF3 promotes the growth of HCC in vivo in a xenograft tumor model. IGSF3 enhances cell cycle progression, cell growth, migration, and invasion of HCC cell lines. Reduction in expression of IGSF3 through knockdown significantly reduces the levels of p-NF-κB/NF-κB and p-IKBα/IKBα, and overexpression of IGSF3 has the opposite result[2].
Technical Parameters
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Species Human
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Source HEK293
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Tag C-10*His
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Accession
O75054-1 (Q20-A1124)
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Molecular Construction
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N-term
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IGSF3 (Q20-A1124)
Accession # O75054 -
His
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C-term
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Protein Length
Extracellular Domain
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Synonyms
IGSF3; Immunoglobulin Superfamily, Member 3; Immunoglobulin Superfamily Member 3; Immunoglobin Superfamily, Member 3; EWI-3; KIAA0466; V8; IgSF3; Glu-Trp-Ile EWI Motif-Containing Protein 3; LCDD; MGC117164; EWI3
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AA Sequence
QRQVTVQEGPLYRTEGSHITIWCNVSGYQGPSEQNFQWSIYLPSSPEREVQIVSTMDSSFPYAIYTQRVRGGKIFIERVQGNSTLLHITDLQARDAGEYECHTPSTDKQYFGSYSAKMNLVVIPDSLQTTAMPQTLHRVEQDPLELTCEVASETIQHSHLSVAWLRQKVGEKPVEVISLSRDFMLHSSSEYAQRQSLGEVRLDKLGRTTFRLTIFHLQPSDQGEFYCEAAEWIQDPDGSWYAMTRKRSEGAVVNVQPTDKEFTVRLETEKRLHTVGEPVEFRCILEAQNVPDRYFAVSWAFNSSLIATMGPNAVPVLNSEFAHREARGQLKVAKESDSVFVLKIYHLRQEDSGKYNCRVTEREKTVTGEFIDKESKRPKNIPIIVLPLKSSISVEVASNASVILEGEDLRFSCSVRTAGRPQGRFSVIWQLVDRQNRRSNIMWLDRDGTVQPGSSYWERSSFGGVQMEQVQPNSFSLGIFNSRKEDEGQYECHVTEWVRAVDGEWQIVGERRASTPISITALEMGFAVTAISRTPGVTYSDSFDLQCIIKPHYPAWVPVSVTWRFQPVGTVEFHDLVTFTRDGGVQWGDRSSSFRTRTAIEKAESSNNVRLSISRASDTEAGKYQCVAELWRKNYNNTWTRLAERTSNLLEIRVLQPVTKLQVSKSKRTLTLVENKPIQLNCSVKSQTSQNSHFAVLWYVHKPSDADGKLILKTTHNSAFEYGTYAEEEGLRARLQFERHVSGGLFSLTVQRAEVSDSGSYYCHVEEWLLSPNYAWYKLAEEVSGRTEVTVKQPDSRLRLSQAQGNLSVLETRQVQLECVVLNRTSITSQLMVEWFVWKPNHPERETVARLSRDATFHYGEQAAKNNLKGRLHLESPSPGVYRLFIQNVAVQDSGTYSCHVEEWLPSPSGMWYKRAEDTAGQTALTVMRPDASLQVDTVVPNATVSEKAAFQLDCSIVSRSSQDSRFAVAWYSLRTKAGGKRSSPGLEEQEEEREEEEEEDDDDDDDPTERTALLSVGPDAVFGPEGSPWEGRLRFQRLSPVLYRLTVLQASPQDTGNYSCHVEEWLPSPQKEWYRLTEEESAPIGIRVLDTSPTLQSIICSNDA
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Molecular Weight
Approximately 140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (242 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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