1. Recombinant Proteins
  2. Cytokines and Growth Factors
  3. Interleukin & Receptors
  4. IL-2 IL-12
  5. IL-12 alpha
  6. IL-12 Protein, Mouse (HEK293, His)

IL-12 Protein, Mouse (HEK293, His)

Cat. No.: HY-P73159
Handling Instructions
Top Publications Citing Use of Products
  • Technical Parameters

  • Properties

  • Documentation

Species

Mouse

Source

HEK 293

Tag

His

Accession

P43431&P43432 (M1-A215&M1-S335)

Gene ID

16159&16160

Synonyms
Interleukin-12 subunit alpha; IL-12A; CLMF p35; IL-12 subunit p35; NKSF1
AA Sequence

Molecular Weight

Approximately 28&47 kDa

Purity

Greater than 90 % as determined by SDS-PAGE

Biological Activity
Measured by its ability to bind recombinant mouse IL12RB2 in a functional ELISA. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells and the ED50 is 2-10 ng/mL.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
IL-12 Protein, Mouse (HEK293, His)
Cat. No.:
HY-P73159
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