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IL-23R Protein, Mouse (HEK293, His)

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IL-23R protein and IL12RB1 form interleukin 23 receptor, which is the key binding site of IL23. IL-23R mediates stimulation of T cells, NK cells, and possibly macrophages/myeloid cells, activating the Jak-Stat signaling cascade. IL-23R Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-23R protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

IL-23R protein and IL12RB1 form interleukin 23 receptor, which is the key binding site of IL23. IL-23R mediates stimulation of T cells, NK cells, and possibly macrophages/myeloid cells, activating the Jak-Stat signaling cascade. IL-23R Protein, Mouse (HEK293, His) is the recombinant mouse-derived IL-23R protein, expressed by HEK293 , with C-His labeled tag.

Background

The IL-23R Protein forms the interleukin-23 receptor in association with IL12RB1. Acting as a binding site for IL23, this receptor mediates stimulation in T-cells, NK cells, and possibly certain macrophage/myeloid cells, likely through the activation of the Jak-Stat signaling cascade. IL-23R plays a crucial role in both innate and adaptive immunity and may contribute to the acute response to infection in peripheral tissues. Additionally, IL-23R has been implicated in autoimmune inflammatory diseases and is thought to be significant in tumorigenesis. Operating as a heterodimer with IL12RB1, the IL-23R complex, when exposed to IL23, forms the functional IL23 receptor. Furthermore, IL-23R interacts with JAK2 and, in the presence of IL23, associates with STAT3, contributing to downstream signaling processes (By similarity).

Verified Bioactivity

Mouse IL-23R, His Tag immobilized on CM5 Chip can bind Mouse IL-23 alpha&IL-12 beta, His Tag with an affinity constant of 6.91 nM as determined in SPR assay (Biacore T200).

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    Q8K4B4 (G24-G374)

  • Gene ID
    209590  [NCBI]
  • Molecular Construction
    • N-term
    • IL-23R (G24-G374)
      Accession # Q8K4B4
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    IL23R; IL-23 Receptor; Interleukin 23 Receptor; IL23RL1-THE1A; Interleukin-23 Receptor; PSORS7; IL-23R

  • AA Sequence

    GITSINCSGDMWVEPGEIFQMGMNVSIYCQEALKHCRPRNLYFYKNGFKEEFDITRINRTTARIWYKGFSEPHAYMHCTAECPGHFQETLICGKDISSGHPPDAPSNLTCVIYEYSGNMTCTWNTGKPTYIDTKYIVHVKSLETEEEQQYLASSYVKISTDSLQGSRKYLVWVQAVNSLGMENSQQLHVHLDDIVIPSASIISRAETTNDTVPKTIVYWKSKTMIEKVFCEMRYKTTTNQTWSVKEFDANFTYVQQSEFYLEPDSKYVFQVRCQETGKRNWQPWSSPFVHQTSQETGKRNWQPWSSPFVHQTSQTVSQVTAKSSHEPQKMEMLSATIFRGHPASGNHQDIG

  • Predicted Molecular Mass

    41.5 kDa

  • Molecular Weight

    Approximately 60-80 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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