IL-25/IL-17E Protein, Mouse (His)

Interleukin-25 (IL-25), also known as IL-17E, belongs to the IL-17 cytokine family. IL-25 activates NF-κB, MAPKs and JAK/STAT signaling pathways^[1]. IL-25 exerts singal transduction through receptors composed of IL17RA and IL17RB^[7]. IL-25/IL-17E Protein, Mouse (His) is the recombinant mouse-derived IL-25/IL-17E protein, expressed by HEK293 , with N-His labeled tag. The total length of IL-25/IL-17E Protein, Mouse (His) is 153 a.a., with molecular weight of 30-35 kDa.

and is also produced by various immune cells such as CD8⁺ T cells, mast cells, macrophages, dendritic cells (DCs), eosinophils, basophils, group 2 innate lymphoid cells (ILC2s), epithelial and endothelial cells^{[1][2][3][4][5][6]}. IL-25 exerts singal transduction through receptors composed of IL17RA and IL17RB^[7]. IL-25 induces the expression of IL-4, IL-5, IL-13, TGF-β, and G-CSF^[8][9]. IL-25 can stimulate cell proliferation, inhibition of apoptosis, production of inflammatory cytokines and chemokines, modulation of cell-cell adhesion, and cell motility in nonimmune cells^{[10][11][12][13]}. IL-25 activates NF-κB, MAPKs and JAK/STATs signaling pathways^[1][14]. The sequence homology of IL-25 between human and mouse, mouse and rat is 78.88% and 91.12% respectively.

1. Measured by its ability to induce CXCL1/GROα secretion in HT29. The ED₅₀ for this effect is 3-14 ng/mL.
2.Immobilized IL17RB Protein, Mouse, Recombinant (hFc Tag) at 2 μg/mL (100 μL/well) can bind IL25/IL17E Protein, Mouse, Recombinant (His Tag),the EC₅₀ is 4-20 ng/mL.

Mouse

HEK293

N-His

Q8VHH8 (V17-A169)

140806  [NCBI]

30-35 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.