1. Recombinant Proteins
  2. Receptor Proteins
  3. Cell Adhesion Molecules (CAMs)
  4. Integrin
  5. Integrin alpha 2B beta 3
  6. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His)

Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His)

Cat. No.: HY-P700754
Handling Instructions

Integrin alpha-IIb/beta-3, a versatile receptor recognizing various ligands, plays a pivotal role in platelet function and hemostasis. It binds fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, and vitronectin through the R-G-D sequence, particularly recognizing the H-H-L-G-G-G-A-K-Q-A-G-D-V sequence in fibrinogen. Activated, it facilitates platelet-platelet interactions by binding soluble fibrinogen, leading to rapid platelet aggregation. Structurally, it forms a heterodimer with the alpha subunit consisting of a heavy and a light chain linked by a disulfide bond, and the alpha-IIb subunit associates with beta-3. Its diverse interactions, including RNF181, CIB1-4, and PPIA/CYPA, underscore its integral role in orchestrating platelet responses. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha 2B beta 3 protein, expressed by HEK293, with C-His labeled tag. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His), has molecular weight of 90-113 kDa.

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Description

Integrin alpha-IIb/beta-3, a versatile receptor recognizing various ligands, plays a pivotal role in platelet function and hemostasis. It binds fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, and vitronectin through the R-G-D sequence, particularly recognizing the H-H-L-G-G-G-A-K-Q-A-G-D-V sequence in fibrinogen. Activated, it facilitates platelet-platelet interactions by binding soluble fibrinogen, leading to rapid platelet aggregation. Structurally, it forms a heterodimer with the alpha subunit consisting of a heavy and a light chain linked by a disulfide bond, and the alpha-IIb subunit associates with beta-3. Its diverse interactions, including RNF181, CIB1-4, and PPIA/CYPA, underscore its integral role in orchestrating platelet responses. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived Integrin alpha 2B beta 3 protein, expressed by HEK293, with C-His labeled tag. Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His), has molecular weight of 90-113 kDa.

Background

The Integrin alpha-IIb/beta-3 protein is a multifaceted receptor with broad ligand recognition capabilities, including binding to fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin, and vitronectin through the R-G-D sequence. Specifically, it recognizes the H-H-L-G-G-G-A-K-Q-A-G-D-V sequence in the fibrinogen gamma chain. Upon activation, Integrin alpha-IIb/beta-3 facilitates platelet-platelet interactions by binding soluble fibrinogen, leading to rapid platelet aggregation that forms a physical plug on the ruptured endothelial cell surface. Structurally, it forms a heterodimer, with the alpha subunit consisting of a heavy and a light chain linked by a disulfide bond, and the alpha-IIb subunit associates with beta-3. Additionally, Integrin alpha-IIb/beta-3 directly interacts with RNF181, and its cytoplasmic tail region interacts with CIB1, CIB2, CIB3, and CIB4 in a calcium and magnesium-dependent manner. Furthermore, its interaction with PPIA/CYPA is ROS and PPIase activity-dependent and is increased in the presence of thrombin. This diverse range of interactions highlights the integral role of Integrin alpha-IIb/beta-3 in platelet function and hemostasis.

Species

Mouse

Source

HEK293

Tag

C-His

Accession

Q9QUM0 (L32-R988)&O54890 (E26-D717)

Gene ID

16399&16416

Synonyms
α2Bβ3; ITGA2B&ITGB3; ITGA2B; ITGB3
Molecular Weight

90-113 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Integrin alpha 2B beta 3 Protein, Mouse (HEK293, His)
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