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Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi)

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ITGA5 is a multifunctional receptor that interacts with multiple ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, human metapneumovirus, and soluble CD40LG. ITGA5 recognizes RGD sequences and mediates cell adhesion through different binding sites. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi), has molecular weight of 100-140 kDa.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi)
  • Experimental Validation Results for Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi)
  • Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
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Biological Activity

Description

ITGA5 is a multifunctional receptor that interacts with multiple ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, human metapneumovirus, and soluble CD40LG. ITGA5 recognizes RGD sequences and mediates cell adhesion through different binding sites. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi), has molecular weight of 100-140 kDa.

Background

The Integrin alpha-5/beta-1 protein (ITGA5:ITGB1) functions as a versatile receptor with diverse ligand interactions. Recognizing the R-G-D sequence in its ligands, ITGA5:ITGB1 serves as a receptor for fibronectin and fibrinogen, mediating cell adhesion through distinct binding sites. Notably, it binds to PLA2G2A at a site separate from its classical ligand-binding site, inducing conformational changes that enhance ligand binding. Additionally, ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1), facilitating R-G-D-dependent cell adhesion. It is also a receptor for fibronectin (FN1), enabling R-G-D-dependent cell adhesion to FN1. Furthermore, ITGA5:ITGB1 serves as a receptor for IL1B, playing a crucial role in IL1B signaling. In the context of microbial infection, ITGA5:ITGB1 acts as a receptor for Human metapneumovirus, highlighting its involvement in pathogen recognition. Moreover, ITGA5:ITGB3 acts as a receptor for soluble CD40LG, playing a vital role in CD40/CD40LG signaling. This broad spectrum of ligand interactions underscores the multifunctionality of ITGA5:ITGB1 in cellular processes and signaling pathways.

Verified Bioactivity

Immobilized Human Integrin alpha 5 beta 1 at 1 μg/mL (100 μL/well) on the plate can bind Integrin alpha 5 beta 1 antibody, the ED50 for this effect is 3-30 ng/mL determined by ELISA.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-Avi;C-8*His
  • Accession

    P08648 (F42-Y995)&P05556-1 (Q21-D728)

  • Gene ID
    3678  [NCBI]&3688  [NCBI]
  • Synonyms

    ITGA5; Integrin Alpha-F; Prev. FNRA; CDNA FLJ50382, Moderately Similar To Integrin Alpha-5; CD49e; Very Late Activation Protein 5, Alpha Subunit; Integrin, Alpha 5 (Fibronectin Receptor, Alpha Polypeptide); Fibronectin Receptor, Alpha Subunit; Fibronectin

  • Predicted Molecular Mass

    111.8 kDa (ITGA5) & 83.2 kDa (ITGB1)

  • Molecular Weight

    Approximately 100-140 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE or Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
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100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Immobilized Human Integrin alpha 5 beta 1 at 1 μg/mL (100 μL/well) on the plate can bind Integrin alpha 5 beta 1 antibody, the ED50 for this effect is 4.554 ng/mL determined by ELISA.

  • Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) Product Image
    1/16
  • Experimental Validation Results for Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi)
  • Experimental Validation Results for Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi)
  • Integrin alpha 5 beta 1 Protein, Human (HEK293, His-Avi) Product Image