Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His)
Based on 1 Customer Validation
Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His) is the recombinant human-derived Integrin alpha 6 beta 1 protein, expressed by HEK293, with C-His, C-Flag labeled tag., has molecular weight of ~110 & 130 kDa, respectively.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Biological Activity
Description
Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His) is the recombinant human-derived Integrin alpha 6 beta 1 protein, expressed by HEK293, with C-His, C-Flag labeled tag., has molecular weight of ~110 & 130 kDa, respectively.
Verified Bioactivity
Measured by its binding ability in a functional ELISA. When Recombinant Human Laminin alpha 4 is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human Integrin alpha 6 beta 1. The ED50 for this effect is 7.473 nM.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His;C-Flag
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Molecular Construction
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N-term
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ITGA6 (F24-S1011)
Accession # P23229-3 -
Flag
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C-term
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N-term
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ITGB4 (Q21-D728)
Accession # P05556-1 -
His
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C-term
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Synonyms
ITGA6; CD49f; Integrin Subunit Alpha 6; Very Late Activation Protein 6; VLA-6; Integrin, Alpha 6; CD49 Antigen-Like Family Member F; Integrin Alpha6B; Integrin Alpha-6; CD49f Antigen; ITGA6A; JEB6; ITGA6B; ITGB1; Very Late Activation Protein, Beta Polypep
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Molecular Weight
Approximately 110 kDa & 130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (232 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)