Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His)

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Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His) is the recombinant human-derived Integrin alpha 6 beta 1 protein, expressed by HEK293, with C-His, C-Flag labeled tag., has molecular weight of ~110 & 130 kDa, respectively.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs

Biological Activity

Description

Integrin alpha 6 beta 1 Protein, Human (HEK293, Flag-His) is the recombinant human-derived Integrin alpha 6 beta 1 protein, expressed by HEK293, with C-His, C-Flag labeled tag., has molecular weight of ~110 & 130 kDa, respectively.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Human Laminin alpha 4 is immobilized at 5 µg/mL (100 µL/well) can bind Biotinylated Recombinant Human Integrin alpha 6 beta 1. The ED50 for this effect is 7.473 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His;C-Flag
  • Accession
  • Gene ID
  • Molecular Construction
    • N-term
    • ITGA6 (F24-S1011)
      Accession # P23229-3
    • Flag
    • C-term
    • N-term
    • ITGB4 (Q21-D728)
      Accession # P05556-1
    • His
    • C-term
  • Synonyms

    ITGA6; CD49f; Integrin Subunit Alpha 6; Very Late Activation Protein 6; VLA-6; Integrin, Alpha 6; CD49 Antigen-Like Family Member F; Integrin Alpha6B; Integrin Alpha-6; CD49f Antigen; ITGA6A; JEB6; ITGA6B; ITGB1; Very Late Activation Protein, Beta Polypep

  • Molecular Weight

    Approximately 110 kDa & 130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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